Research Article
Applications Of Lipase Enzyme In Fatty Monoethanolamide Synthesis Based On Palm Fatty Acid Distillate
@INPROCEEDINGS{10.4108/eai.20-1-2018.2282449, author={Fachraniah Ahmad and Eka Kurniasih and Faridah Faridah and Ariefin Ariefin}, title={Applications Of Lipase Enzyme In Fatty Monoethanolamide Synthesis Based On Palm Fatty Acid Distillate}, proceedings={Proceedings of the 1st Workshop on Multidisciplinary and Its Applications Part 1, WMA-01 2018, 19-20 January 2018, Aceh, Indonesia}, publisher={EAI}, proceedings_a={WMA-1}, year={2019}, month={9}, keywords={amidation enzymes pfad monoethanolamine rhizomucor meihei}, doi={10.4108/eai.20-1-2018.2282449} }- Fachraniah Ahmad
Eka Kurniasih
Faridah Faridah
Ariefin Ariefin
Year: 2019
Applications Of Lipase Enzyme In Fatty Monoethanolamide Synthesis Based On Palm Fatty Acid Distillate
WMA-1
EAI
DOI: 10.4108/eai.20-1-2018.2282449
Abstract
Monoethanolamide is a compound of nonionic surfactant which can be obtained through a reaction between Palm Fatty Acid Distillate (PFAD) with alkanolamine. It has OH- group, so it dissolves easily in water and is widely used in the cosmetics industry. The screening of enzyme was conducted in temperature of 600C and the ratio of biocatalyst 0.2 % (w/w) in three types of lipase enzymes, which are Rhizomucor meihei, Candida antartica and Lipase sp. The determination of mole ratio level for PFAD/ethanolamine was conducted in four hour reaction times. The level of mole ratio of PFAD/ethanolamine was varied from 1:1 up to 1:10. Crude fatty monoethanolamide which is soluted in alcohol was separated from biocatalyst by using filtration method and carried on with distillation process to separate the ethanol from the product. Pure fatty monoethanolamide was gained as bottom product. Monoethanolamide was then confirmed in infra red spectrum (FT-IR). From the results of enzymes screening, it is known that Rhizomucor meihei is a type of enzyme that is able to hold the amidation reaction to provide the highest product conversion of 29.51% compared to other types of enzymes. While the increase in the ratio of moles PFAD/monoethanolamine gives the best results on the 1:4, and the conversion obtained is 86.25%.
