Research Article
In vitro chronic wound healing using collagen and plant extract along with zinc nanoparticles
@ARTICLE{10.4108/eetpht.10.5784, author={J Sofia Bobby and S Purnima and V Mythily and B Ghiri Rajan and S Shubhankar and M Sowmiya}, title={In vitro chronic wound healing using collagen and plant extract along with zinc nanoparticles}, journal={EAI Endorsed Transactions on Pervasive Health and Technology}, volume={10}, number={1}, publisher={EAI}, journal_a={PHAT}, year={2024}, month={4}, keywords={Type-I collagen, Cassia fistula, Zinc nanoparticles, Anti-Bacterial culture, MIT assay, Biofilm}, doi={10.4108/eetpht.10.5784} }
- J Sofia Bobby
S Purnima
V Mythily
B Ghiri Rajan
S Shubhankar
M Sowmiya
Year: 2024
In vitro chronic wound healing using collagen and plant extract along with zinc nanoparticles
PHAT
EAI
DOI: 10.4108/eetpht.10.5784
Abstract
INTRODUCTION: While the use of zinc nanoparticles (ZnNPs) as an antibacterial agent in the biomedical industry has recently attracted significant attention, collagen has aroused significant interest as a biomaterial in medical and tissue engineering applications. OBJECTIVES: In order to create biofilm loaded with biosynthesized ZnNPs for use in chronic wound healing applications, type-I collagen was extracted from the study's subject. by the acid soluble collagen technique, collagen was isolated from the fish skin of the trevally and identified by SDS-PAGE. Aqueous extract from Cassia fistula leaves was also used to greenly manufacture stable ZnNPs, which were then characterized by UV-Vis, FTIR, and XRD measurements. METHODS: Collagen and ZnNPs were then added to polyvinyl alcohol (PVA), creating a thin biofilm that had a high biocompatibility due to the production method's absence of a chemical reducer and crosslinking agent. When tested against the harmful bacteria, both ZnNPs alone and PVA/Collagen/ZnNPs biofilms showed potent antibacterial activity. RESULTS: By using the MTT test, the cytotoxic effects of collagen and ZnNPs on the Vero cell line were evaluated. With 97.76% wound closure, the PVA/Collagen/ZnNPs biofilm demonstrated strong in vitro wound scratch healing efficacy. CONCLUSION: The findings show that the PVA/Collagen/ZnNPs film dramatically increased cell migration by 40.0% at 24 hours, 79.20% at 48 hours, and 97.76% at 74 hours.
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