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Research Article

In vitro chronic wound healing using collagen and plant extract along with zinc nanoparticles

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  • @ARTICLE{10.4108/eetpht.10.5784,
        author={J Sofia Bobby and S Purnima and V Mythily and B Ghiri Rajan and S Shubhankar and M Sowmiya},
        title={In vitro chronic wound healing using collagen and plant extract along with zinc nanoparticles},
        journal={EAI Endorsed Transactions on Pervasive Health and Technology},
        volume={10},
        number={1},
        publisher={EAI},
        journal_a={PHAT},
        year={2024},
        month={4},
        keywords={Type-I collagen, Cassia fistula, Zinc nanoparticles, Anti-Bacterial culture, MIT assay, Biofilm},
        doi={10.4108/eetpht.10.5784}
    }
    
  • J Sofia Bobby
    S Purnima
    V Mythily
    B Ghiri Rajan
    S Shubhankar
    M Sowmiya
    Year: 2024
    In vitro chronic wound healing using collagen and plant extract along with zinc nanoparticles
    PHAT
    EAI
    DOI: 10.4108/eetpht.10.5784
J Sofia Bobby1,*, S Purnima1, V Mythily1, B Ghiri Rajan1, S Shubhankar1, M Sowmiya1
  • 1: Jerusalem College of Engineering
*Contact email: sofiabobbyj@jerusalemengg.ac.in

Abstract

INTRODUCTION: While the use of zinc nanoparticles (ZnNPs) as an antibacterial agent in the biomedical industry has recently attracted significant attention, collagen has aroused significant interest as a biomaterial in medical and tissue engineering applications. OBJECTIVES: In order to create biofilm loaded with biosynthesized ZnNPs for use in chronic wound healing applications, type-I collagen was extracted from the study's subject. by the acid soluble collagen technique, collagen was isolated from the fish skin of the trevally and identified by SDS-PAGE. Aqueous extract from Cassia fistula leaves was also used to greenly manufacture stable ZnNPs, which were then characterized by UV-Vis, FTIR, and XRD measurements. METHODS: Collagen and ZnNPs were then added to polyvinyl alcohol (PVA), creating a thin biofilm that had a high biocompatibility due to the production method's absence of a chemical reducer and crosslinking agent. When tested against the harmful bacteria, both ZnNPs alone and PVA/Collagen/ZnNPs biofilms showed potent antibacterial activity. RESULTS: By using the MTT test, the cytotoxic effects of collagen and ZnNPs on the Vero cell line were evaluated. With 97.76% wound closure, the PVA/Collagen/ZnNPs biofilm demonstrated strong in vitro wound scratch healing efficacy. CONCLUSION: The findings show that the PVA/Collagen/ZnNPs film dramatically increased cell migration by 40.0% at 24 hours, 79.20% at 48 hours, and 97.76% at 74 hours.

Keywords
Type-I collagen, Cassia fistula, Zinc nanoparticles, Anti-Bacterial culture, MIT assay, Biofilm
Received
2024-01-02
Accepted
2024-04-10
Published
2024-04-15
Publisher
EAI
http://dx.doi.org/10.4108/eetpht.10.5784

Copyright © 2024 J. S. Bobby et al., licensed to EAI. This is an open access article distributed under the terms of the CC BY-NC-SA 4.0, which permits copying, redistributing, remixing, transformation, and building upon the material in any medium so long as the original work is properly cited.

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