Evaluation the Diagnostic Values of Direct and Indirect Non-invasive Biochemical Markers of Liver Fibrosis in Patients with Chronic Hepatitis B Virus

Liver fibrosis is the production of an excessively high amount of scar tissue in the liver It occurs when the liver attempts to repair and replace damaged cells, many conditions can damage the liver. Fibrosis itself causes no symptoms, but severe scarring can lead to cirrhosis. the persist of liver viral infection causing chronic injury and led to excessive production of extracellular matrix by hepatic stellate cells in the liver, and finally may cause liver cirrhosis and cancer. To study the fibrosis of the liver in chronic hepatitis B patients by non-invasive direct and indirect biomarkers and evaluate the diagnostic efficiency of these biomarkers. A case-control study involves 119 participants: 50 healthy persons as control and 69 patients with chronic hepatitis B, who were diagnosed by PCR technique. Assessment of the direct biomarkers (serum fibronectin, Haptoglobin, and TNF alpha) and indirect markers (serum total bilirubin, direct bilirubin, aspartate aminotransferase AST, alanine aminotransferase ALT, alkaline phosphatase ALP, albumin, and gamma-glutamyl transferase GGT), in addition to cholesterol. Comparison between control and HBV patients showed a significant difference (P-Value <0.05) for liver function tests and fibrotic marker: fibronectin (168.56± 98.47 control vs. 98.24 ± 74.87 HBV ) and TNF alpha (121.96+124.8 control vs. 56.03±48.39 HBV). The haptoglobin and cholesterol revealed no differences between males or females when compared with control.