Research Article
Comparative Meta-Transcriptome Analysis ofArabidopsis thaliana Under Organic Stress and Its Validation
@INPROCEEDINGS{10.4108/eai.7-12-2021.2314745, author={Rajaseetharama Sekar and Hema Jagadeesan}, title={Comparative Meta-Transcriptome Analysis ofArabidopsis thaliana Under Organic Stress and Its Validation}, proceedings={Proceedings of the First International Conference on Combinatorial and Optimization, ICCAP 2021, December 7-8 2021, Chennai, India}, publisher={EAI}, proceedings_a={ICCAP}, year={2021}, month={12}, keywords={plant-microbe interaction; organic stress; root transcriptome; root exudates;arabidopsis thaliana; rhizo-remediation; meta-analysis of root transcriptome}, doi={10.4108/eai.7-12-2021.2314745} }- Rajaseetharama Sekar
Hema Jagadeesan
Year: 2021
Comparative Meta-Transcriptome Analysis ofArabidopsis thaliana Under Organic Stress and Its Validation
ICCAP
EAI
DOI: 10.4108/eai.7-12-2021.2314745
Abstract
Globalization and industrialization had led to the production of large quantities of waste with complex contents; these are prone to cause serious impact on sustainable development. Plant-microbe integrated system was found to be effective in treating specific waste streams in constructed wetlands or in rhizo-remediation. The molecular basis for this effective treatment vis-à-vis the interaction between plant and microbe in any such bio-augmented phytoremediation system is not well understood. Towards this end, a meta-analysis of transcriptomes of the model plantArabidopsis thaliana (Col-0) was performed by analysing 36 microarray samples belonging to seven microarray studies under various organic stresses. the genes involved in metabolic pathways of the root exudates were analysed. Totally 1313 genes involved in 26 metabolic pathways that in-turn corresponds to 55 root exudates were identified. Comparison of the DEG list of all stress conditions and the genes involved in root exudates, resulted in the short listing of four candidate genes that were highly influential both, to validate this in-silico result, a real time experimental set-up of Arabidopsis thaliana(Col-0) under simulated dye and microbial stress was designed. After treatment, Real Time Quantitative PCR was done for the four candidate genes and the expression pattern of those genes in real time conditions was obtained to corroborate thein-silico result.
