Research Article
DNA Barcoding of Clown Loach Chromobotia macracanthus from Ogan and Musi River Based on Cytochrome C Oxidase Subunit I (COI) Gene
@INPROCEEDINGS{10.4108/eai.5-10-2022.2328352, author={Risky Nur Aulia Pratama and Mochamad Syaifudin and Marini Wijayanti}, title={DNA Barcoding of Clown Loach Chromobotia macracanthus from Ogan and Musi River Based on Cytochrome C Oxidase Subunit I (COI) Gene}, proceedings={Proceedings of the 3rd Sriwijaya International Conference on Environmental Issues, SRICOENV 2022, October 5th, 2022, Palembang, South Sumatera, Indonesia}, publisher={EAI}, proceedings_a={SRICOENV}, year={2023}, month={4}, keywords={tiger botia coi gene phylogenetic}, doi={10.4108/eai.5-10-2022.2328352} }- Risky Nur Aulia Pratama
Mochamad Syaifudin
Marini Wijayanti
Year: 2023
DNA Barcoding of Clown Loach Chromobotia macracanthus from Ogan and Musi River Based on Cytochrome C Oxidase Subunit I (COI) Gene
SRICOENV
EAI
DOI: 10.4108/eai.5-10-2022.2328352
Abstract
The clown loach Chromobotia macracanthus, also known as the tiger botia, is a species of ornamental fish included in the tribe Cobtidae. The tiger botia is native to Indonesia in Batanghari Jambi, and the Musi-Ogan watershed in South Sumatra. Genetic studies of tiger botia from Sumatra with DNA barcoding are needed for genetic conservation. This study aims to determine the nucleotide sequence of COI gene, genetic distance and provide a phylogenetic of tiger botia. These research steps were DNA isolation, DNA amplification using PCR, and sequencing of the COI gene. The results indicated that the size of DNA band was 638 bp. BLASTn showed that LK 2,3,4,5 and SK 1,2,3,5 had the highest similarity of 98.80% with Indian C. macracanthus, except for LK 3, which had 98.11%. The genetic distance was 0.000 and the phylogenetics tree had a bootstrap value of 98% againts the same genus of C. macracanthus. The water quality parameters of 2 rivers i.e, temperature in the range of 29.8-31.1°C, pH 5.50-7.20, dissolved oxygen 5.47-6.76 mg L-1, ammonia 0.0189-0.2115 mg L-1, TDS 0.012- 0.172 mg L-1, salinity 0.5 ppt, total alkalinity 8-18 ppm, water transparency 20-38.5 cm, and current velocity 2.9-10.2 cm s-1.