Proceedings of the 3rd Sriwijaya International Conference on Environmental Issues, SRICOENV 2022, October 5th, 2022, Palembang, South Sumatera, Indonesia

Research Article

The Effect of Several Sterilization Techniques in String Bean Tissue Culture

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  • @INPROCEEDINGS{10.4108/eai.5-10-2022.2328348,
        author={Irmawati  Irmawati and Firdaus  Sulaiman and Zaidan P. Negara and Marlina  Marlina and Lidwina N. Sulistyaningsih and Dinda  Asari and Laras Indah Lestari},
        title={The Effect of Several Sterilization Techniques in String Bean Tissue Culture},
        proceedings={Proceedings of the 3rd Sriwijaya International Conference on Environmental Issues, SRICOENV 2022, October 5th, 2022, Palembang, South Sumatera, Indonesia},
        publisher={EAI},
        proceedings_a={SRICOENV},
        year={2023},
        month={4},
        keywords={effect sterilization techniques string bean tissue culture},
        doi={10.4108/eai.5-10-2022.2328348}
    }
    
  • Irmawati Irmawati
    Firdaus Sulaiman
    Zaidan P. Negara
    Marlina Marlina
    Lidwina N. Sulistyaningsih
    Dinda Asari
    Laras Indah Lestari
    Year: 2023
    The Effect of Several Sterilization Techniques in String Bean Tissue Culture
    SRICOENV
    EAI
    DOI: 10.4108/eai.5-10-2022.2328348
Irmawati Irmawati1,*, Firdaus Sulaiman1, Zaidan P. Negara1, Marlina Marlina1, Lidwina N. Sulistyaningsih1, Dinda Asari1, Laras Indah Lestari1
  • 1: Department of Agronomy, Faculty of Agriculture, Universitas Sriwijaya.
*Contact email: irmawati@fp.unsri.ac.id

Abstract

Tissue culture is a plant propagation technique in which plant parts such as cells, tissues, organs or the whole plant are isolated under aseptic conditions. The aim of this study is to find the best sterilization technique for tissue culture of string beans. This study was conducted in tissue culture laboratory of Faculty of Agriculture, Universitas Sriwijaya. The treatments consisted of 6 sterilization methods, each of which consisted of a combination of different sterilizing agents (fungicide, detergent, bleach solution, and alcohol) and the duration of sterilization. Based on the results, it was found that treatment S4 with a 0.2% fungicide for 15 minutes, followed by a detergent for 3 minutes, a 20% bleach solution (with a sodium hypochlorite active ingredient of 5.25%) for 20 minutes, and 70% alcohol for 10 minutes provided the best results, as evidenced by a relatively lower percentage of contamination and a higher percentage of live explants compared to the other treatments.