Research Article
Prospect of Agricultural waste as media propagation of Bacillus thuringiensis and its pathogenicity against Oryctes rhinoceros larvae
@INPROCEEDINGS{10.4108/eai.5-10-2022.2328335, author={Yulia Pujiastuti and Abu Umayah and Suparman Suparman and Weri Herlin}, title={Prospect of Agricultural waste as media propagation of Bacillus thuringiensis and its pathogenicity against Oryctes rhinoceros larvae}, proceedings={Proceedings of the 3rd Sriwijaya International Conference on Environmental Issues, SRICOENV 2022, October 5th, 2022, Palembang, South Sumatera, Indonesia}, publisher={EAI}, proceedings_a={SRICOENV}, year={2023}, month={4}, keywords={agricultural waste bacillus thuringiensis pathogenicity oryctes rhinoceros larvae}, doi={10.4108/eai.5-10-2022.2328335} }- Yulia Pujiastuti
Abu Umayah
Suparman Suparman
Weri Herlin
Year: 2023
Prospect of Agricultural waste as media propagation of Bacillus thuringiensis and its pathogenicity against Oryctes rhinoceros larvae
SRICOENV
EAI
DOI: 10.4108/eai.5-10-2022.2328335
Abstract
Bacillus thuringiensis has been recognized as a prospective bio-insecticide. Various media are used to propagate B. thuringiensis and expected to be used to control insect pests. Oryctes rhinoceros (Coleoptera: Scarabaeidae) as one of the important pests in oil palm plantations must be controlled in an environmentally friendly and sustainable way. The aim of this research was to study various agricultural waste media in propagation of B. thuringiensis bacteria and their pathogenicity against O rhinoceros larvae. The experimental design was a completely randomized design (CRD) with 7 treatments and 4 replications. Observations were mainly carried out on the density of B. thuringiensis spores propagated in various types of agricultural waste, the pathogenicity of B. thuringiensis and the effect of treatment on Oryctes body length. Experimental results showed after 72 hours of fermentation, the highest spore density was found in the treatment of a mixture of rice bran water and biourine (1:1). The highest pathogenicity of this mixture reached 91.67%, as well as on the treatment of coconut water and 5% molasses. The body length of Oryctes decreased from day 12th. The use of B. thuringiensis bacteria has an opportunity to be used as biological control agents.