The 3rd International Conference Community Research and Service Engagements, IC2RSE 2019, 4th December 2019, North Sumatra, Indonesia

Research Article

Identification Genetic of Soybean Mutant (Glycine max L. Merril) Based on Fatty Acid Characters Using Simple Sequence Repeat (SSR) Markers

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  • @INPROCEEDINGS{10.4108/eai.4-12-2019.2293861,
        author={Hafizha Hayati Barus and Eva Sartini Bayu and Diana Sofia Hanafiah},
        title={Identification Genetic of Soybean Mutant (Glycine max L. Merril) Based on Fatty Acid Characters Using Simple Sequence Repeat (SSR) Markers},
        proceedings={The 3rd International Conference Community Research and Service Engagements, IC2RSE 2019, 4th December  2019, North Sumatra, Indonesia},
        publisher={EAI},
        proceedings_a={IC2RSE},
        year={2020},
        month={4},
        keywords={mutant soybean fatty acid ssr markers},
        doi={10.4108/eai.4-12-2019.2293861}
    }
    
  • Hafizha Hayati Barus
    Eva Sartini Bayu
    Diana Sofia Hanafiah
    Year: 2020
    Identification Genetic of Soybean Mutant (Glycine max L. Merril) Based on Fatty Acid Characters Using Simple Sequence Repeat (SSR) Markers
    IC2RSE
    EAI
    DOI: 10.4108/eai.4-12-2019.2293861
Hafizha Hayati Barus1,*, Eva Sartini Bayu1, Diana Sofia Hanafiah1
  • 1: Agroteknologi Departement, Faculty of Agriculture, Universitas Sumatera Utara
*Contact email: hafizha.hhb@gmail.com

Abstract

Soybean is one of the leading strategic food crop in Indonesia. Nowadays, people's need for soybeans is not only seen from the production aspects but also soybean nutrient contents, especially fatty acids. One of the ways to develop superior varieties of soybean with high fatty acids is through mutations. Seven soybean mutants selected M5 and M7 generation with high production character and resistance Athelia rolfsii Curz were genetically related to high fatty acids using SSR markers. DNA quality and quantity test results showed DNA purity between 1.12-2.13 with concentration between 15.4-97.9 μg/mL. The FAD2-2D locus is the gene candidate of the result by the QTL mapping used in this study to identify soybean genes related to linoleic acid characters. The results showed that the primer used was specific and polymorphic. Mutant soybeans tested have a high genetic diversity.