The 3rd International Conference Community Research and Service Engagements, IC2RSE 2019, 4th December 2019, North Sumatra, Indonesia

Research Article

The Therapeutic Effect of Nigella Sativa Extract on Female Wistar Rats Vaginal Candidiasis Model

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  • @INPROCEEDINGS{10.4108/eai.4-12-2019.2293816,
        author={Muhammad  Rusda and M. Fidel Ganis Siregar and Aznan  Lelo and Syafruddin  Ilyas and Ratna Akbari Ganie and H.Kms. Yusuf Effendi and Poppy  Anjelisa and R Lia Iswara},
        title={The Therapeutic Effect of Nigella Sativa Extract on Female Wistar Rats Vaginal Candidiasis Model},
        proceedings={The 3rd International Conference Community Research and Service Engagements, IC2RSE 2019, 4th December  2019, North Sumatra, Indonesia},
        publisher={EAI},
        proceedings_a={IC2RSE},
        year={2020},
        month={4},
        keywords={fungal inoculation pseudoestrus candida albicans nigella sativa vaginal},
        doi={10.4108/eai.4-12-2019.2293816}
    }
    
  • Muhammad Rusda
    M. Fidel Ganis Siregar
    Aznan Lelo
    Syafruddin Ilyas
    Ratna Akbari Ganie
    H.Kms. Yusuf Effendi
    Poppy Anjelisa
    R Lia Iswara
    Year: 2020
    The Therapeutic Effect of Nigella Sativa Extract on Female Wistar Rats Vaginal Candidiasis Model
    IC2RSE
    EAI
    DOI: 10.4108/eai.4-12-2019.2293816
Muhammad Rusda1,*, M. Fidel Ganis Siregar1, Aznan Lelo2, Syafruddin Ilyas3, Ratna Akbari Ganie4, H.Kms. Yusuf Effendi5, Poppy Anjelisa6, R Lia Iswara7
  • 1: Obstetric and Gynecology Department, Faculty of Medicine, Universitas Sumatera Utara
  • 2: Pharmacology Department, Faculty of Medicine, Universitas Sumatera Utara
  • 3: Biology Departement, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara
  • 4: Clinical Pahology Depatment, Faculty of Medicine, Universitas Sumatera Utara
  • 5: Obstetric and Gynecology Department, Faculty of Medicine, Universitas Sriwijaya
  • 6: Faculty of Pharmacy, Universitas Sumatera Utara
  • 7: Microbiology Department, Faculty of Medicine, Universitas Sumatera Utara
*Contact email: mrusdaharahap@yahoo.com

Abstract

Candidiasis is the most common fungal infection of humans caused by Candida albicans. Immediate and proper management of vaginitis candida must be done to prevent complications. Nigella sativa has pharmacological effects as antimicrobial, anti-inflammatory, immune stimulation and anti-cancer properties. Nigella sativa was proven have antifungal effect in vitro, and this study was to assess the antifungal effects and possibility of in vivo therapy. This study is an analytical study that assesses the therapeutic effect of Nigella sativa in a rat vaginal candidiasis model. Subjects were 28 rats that had been inoculated with Candida albicans and divided into 4 groups: Nigella sativa group, fluconazole group, combination of Nigella sativa with fluconazole group and control group. Candida albicans colony was measured to assess the therapeutic effect of the treatment. There were no difference number of Candida albicans colony between all group before inoculation (p = 0,274) and after inoculation (p = 0,323). There were significant decreased number of Candida albicans colony on the 24 hours after the treatment between the three types of treatment with the control group (Nigella sativa group p = 0,046; Fluconazole group p = 0,002; Nigella sativa + fluconazole p = 0,002). There was therapeutic effect of Nigella sativa by reducing the number of Candida albicans colonies.