Proceedings of the 1st International Conference on Education, Humanities, Health and Agriculture, ICEHHA 2021, 3-4 June 2021, Ruteng, Flores, Indonesia

Research Article

Bacterial Diversity on the Application of Methanotrophic Bacteria in Rainfed Rice of Kupang Regency, East Nusa Tenggara, Indonesia

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  • @INPROCEEDINGS{10.4108/eai.3-6-2021.2310761,
        author={Rizki Adiputra Taopan and Iman  Rusmana and Dwi Andreas Santosa},
        title={Bacterial Diversity on the Application of Methanotrophic Bacteria in Rainfed Rice of Kupang Regency, East Nusa Tenggara, Indonesia },
        proceedings={Proceedings of the 1st International Conference on Education, Humanities, Health and Agriculture, ICEHHA 2021, 3-4 June 2021, Ruteng, Flores, Indonesia},
        publisher={EAI},
        proceedings_a={ICEHHA},
        year={2021},
        month={8},
        keywords={dgge; methanotroph; methane emission; rainfed rice},
        doi={10.4108/eai.3-6-2021.2310761}
    }
    
  • Rizki Adiputra Taopan
    Iman Rusmana
    Dwi Andreas Santosa
    Year: 2021
    Bacterial Diversity on the Application of Methanotrophic Bacteria in Rainfed Rice of Kupang Regency, East Nusa Tenggara, Indonesia
    ICEHHA
    EAI
    DOI: 10.4108/eai.3-6-2021.2310761
Rizki Adiputra Taopan1,*, Iman Rusmana2, Dwi Andreas Santosa3
  • 1: Faculty of Health Science and Agricultural, Indonesian Catholic University of St Paul Ruteng, Indonesia
  • 2: Faculty of Mathematics and Natural Resources, IPB University, Indonesia
  • 3: Faculty of Agriculture, IPB University, Indonesia
*Contact email: rizkimicro@gmail.com

Abstract

Paddy fields are the main anthropogenic source of methane emissions as a greenhouse gas that causes global warming. In this research, methanotrophic bacteria as biological fertilizers are used to reduce methane gas in rainfed rice fields and study the diversity of bacteria based on the application of these fertilizers. This research began with the cultured of the bacterial cultures of Methylocystis rosea BGM 1, Methylocystis palvus BGM 3, M. capsulatus BGM 9 and Methylobacter sp. SKM 14. Furthermore, culture production and application are carried out in rainfed rice fields. The treatments consisted of: (1) NPK 100% (P1), (2) NPK 50% (P2), (3) without fertilizer application (P3), (4) NPK 100% + Metanotrophs (P4), (5) NPK 50% + Metanotrophs (P5), and (6) Metanotrophs (P6). Analysis of the diversity of bekteri using the Deanaturing Gradient Gel Eltrophoresis (DGGE) technique. The study of bacterial diversity based on the 16S rRNA gene showed that the proteobacteria genus dominated the habitat consisting of the gammaproteobacteria, alfaproteobacteria and deltaproteobacteria classes.