Research Article
Optimization of Cellulase Production by Chaetomium globosum 17BDSM Using Solid Phase Fermentation Method
@INPROCEEDINGS{10.4108/eai.3-11-2023.2347973, author={Elisa Nurnawati and Hary Widjajanti and Muharni Muharni and Rasti Puspha Amrina Rosyada}, title={Optimization of Cellulase Production by Chaetomium globosum 17BDSM Using Solid Phase Fermentation Method}, proceedings={Proceedings of the 3rd Sriwijaya International Conference on Basic and Applied Sciences, SICBAS 2023, November 3, 2023, Palembang, Indonesia}, publisher={EAI}, proceedings_a={SICBAS}, year={2024}, month={8}, keywords={cellulose cellulase chaetomium globosum optimization}, doi={10.4108/eai.3-11-2023.2347973} }- Elisa Nurnawati
Hary Widjajanti
Muharni Muharni
Rasti Puspha Amrina Rosyada
Year: 2024
Optimization of Cellulase Production by Chaetomium globosum 17BDSM Using Solid Phase Fermentation Method
SICBAS
EAI
DOI: 10.4108/eai.3-11-2023.2347973
Abstract
Cellulose is still present in agricultural byproducts. Fungi can use cellulose as a source of carbon and energy as well as a substrate for cellulase production. Chaetomium globosum 17BDSM is a fungus with cellulolytic properties. Cellulase activity is influenced by the following factors: pH, temperature, substrate thickness, and substrate type. This study aims to find the optimal substrate thickness, pH, and temperature for C. globosum 17BDSM growth in order to produce high-activity cellulase. A factorial Randomised Block Design experimental design was used in this study to treat substrate type, temperature, pH, and substrate thickness. According to the findings, thickness affects cellulase activity; at 1.5 cm of thickness, cellulase activity is 0.154 U/mL. The substrate type affects cellulase activity, notably sugarcane bagasse and corncob, which had cellulase activity values of 0.321 U/mL and 0.040 U/mL, respectively. Both temperature and pH treatments showed no influence on cellulase activity.