Research Article
Testing the Inhibition Activity of the Alpha-Glucosidase Enzyme of Melinjo Leaf (Gnetum gnemon L.) Extract and Fractions in Vitro
@INPROCEEDINGS{10.4108/eai.3-11-2023.2347958, author={Herlina Herlina and Elfita Elfita and Irsan Saleh and Mardiyanto Mardiyanto}, title={Testing the Inhibition Activity of the Alpha-Glucosidase Enzyme of Melinjo Leaf (Gnetum gnemon L.) Extract and Fractions in Vitro}, proceedings={Proceedings of the 3rd Sriwijaya International Conference on Basic and Applied Sciences, SICBAS 2023, November 3, 2023, Palembang, Indonesia}, publisher={EAI}, proceedings_a={SICBAS}, year={2024}, month={8}, keywords={melinjo leaf (gnetum gnemon l) α-glucosidase enzyme ic50 n-hexane fraction}, doi={10.4108/eai.3-11-2023.2347958} }- Herlina Herlina
Elfita Elfita
Irsan Saleh
Mardiyanto Mardiyanto
Year: 2024
Testing the Inhibition Activity of the Alpha-Glucosidase Enzyme of Melinjo Leaf (Gnetum gnemon L.) Extract and Fractions in Vitro
SICBAS
EAI
DOI: 10.4108/eai.3-11-2023.2347958
Abstract
High blood sugar levels are one of the metabolic disorders associated with diabetes mellitus. Oral antidiabetic therapy using α-glucosidase inhibitor agents. By blocking the α-glucosidase enzyme, the research aims to ascertain the antidiabetic potential of melinjo leaf (Gnetum gnemon L.) extracts and fractions. The inhibition test was carried out by using a microplate reader to measure the absorbance of p-nitrophenol produced as a product of the enzymatic reaction at a wavelength of 405 nm. Ethanol extract, ethanol fraction, ethyl acetate fraction, and n-hexane fraction had IC50 values of 589.39, 494.51, 99.61, and 56.82, respectively. The α-glucosidase enzyme is more likely to be inhibited by the ethyl acetate and n-hexane fractions than by the ethanol extract and ethanol fraction. According to phytochemical analyses, the n-hexane fraction contained steroids while the ethyl acetate fraction contained flavonoids, polyphenols, tannins, and saponins. The αglucosidase enzyme may be inhibited by the n-hexane and ethyl acetate fractions.
