Testing the Inhibition Activity of the Alpha-Glucosidase Enzyme of Melinjo Leaf (Gnetum gnemon L.) Extract and Fractions in Vitro

Herlina Herlina; Elfita Elfita; Irsan Saleh; Mardiyanto Mardiyanto

Proceedings of the 3rd Sriwijaya International Conference on Basic and Applied Sciences, SICBAS 2023, November 3, 2023, Palembang, Indonesia

Research Article

Testing the Inhibition Activity of the Alpha-Glucosidase Enzyme of Melinjo Leaf (Gnetum gnemon L.) Extract and Fractions in Vitro

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@INPROCEEDINGS{10.4108/eai.3-11-2023.2347958,
    author={Herlina  Herlina and Elfita  Elfita and Irsan  Saleh and Mardiyanto  Mardiyanto},
    title={Testing the Inhibition Activity of the Alpha-Glucosidase Enzyme of Melinjo Leaf (Gnetum gnemon L.) Extract and Fractions in Vitro},
    proceedings={Proceedings of the 3rd Sriwijaya International Conference on Basic and Applied Sciences, SICBAS 2023, November 3, 2023, Palembang, Indonesia},
    publisher={EAI},
    proceedings_a={SICBAS},
    year={2024},
    month={8},
    keywords={melinjo leaf (gnetum gnemon l) α-glucosidase enzyme ic50 n-hexane fraction},
    doi={10.4108/eai.3-11-2023.2347958}
}

Herlina Herlina
Elfita Elfita
Irsan Saleh
Mardiyanto Mardiyanto
Year: 2024
Testing the Inhibition Activity of the Alpha-Glucosidase Enzyme of Melinjo Leaf (Gnetum gnemon L.) Extract and Fractions in Vitro
SICBAS
EAI
DOI: 10.4108/eai.3-11-2023.2347958

Herlina Herlina¹^,*, Elfita Elfita², Irsan Saleh³, Mardiyanto Mardiyanto¹

1: Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Sriwijaya of University
2: Department of Chemistry, Faculty of Mathematics and Natural Sciences, Sriwijaya of University
3: Department of Medical, Faculty of Medical, Sriwijaya of University

*Contact email: herlinafarmasi@mipa.unsri.ac.id

Abstract

High blood sugar levels are one of the metabolic disorders associated with diabetes mellitus. Oral antidiabetic therapy using α-glucosidase inhibitor agents. By blocking the α-glucosidase enzyme, the research aims to ascertain the antidiabetic potential of melinjo leaf (Gnetum gnemon L.) extracts and fractions. The inhibition test was carried out by using a microplate reader to measure the absorbance of p-nitrophenol produced as a product of the enzymatic reaction at a wavelength of 405 nm. Ethanol extract, ethanol fraction, ethyl acetate fraction, and n-hexane fraction had IC50 values of 589.39, 494.51, 99.61, and 56.82, respectively. The α-glucosidase enzyme is more likely to be inhibited by the ethyl acetate and n-hexane fractions than by the ethanol extract and ethanol fraction. According to phytochemical analyses, the n-hexane fraction contained steroids while the ethyl acetate fraction contained flavonoids, polyphenols, tannins, and saponins. The αglucosidase enzyme may be inhibited by the n-hexane and ethyl acetate fractions.

Keywords: melinjo leaf (gnetum gnemon l) α-glucosidase enzyme ic50 n-hexane fraction

Published: 2024-08-02
Publisher: EAI

: http://dx.doi.org/10.4108/eai.3-11-2023.2347958