Research Article
Sungkai (Peronema canescens Jack) Leaf Methanol Extract for Proliferation of Saccharomyces cerevisiae Yeast and Mice (Mus muculus)
@INPROCEEDINGS{10.4108/eai.3-11-2023.2347957, author={Irena Francina Kelly Kamal and Hermansyah Hermansyah and Desnelli Desnelli and Muharni Muharni and Yosie Andriani}, title={Sungkai (Peronema canescens Jack) Leaf Methanol Extract for Proliferation of Saccharomyces cerevisiae Yeast and Mice (Mus muculus)}, proceedings={Proceedings of the 3rd Sriwijaya International Conference on Basic and Applied Sciences, SICBAS 2023, November 3, 2023, Palembang, Indonesia}, publisher={EAI}, proceedings_a={SICBAS}, year={2024}, month={8}, keywords={sungkai leukocytes s cerevisiae proliferation immunomodulator}, doi={10.4108/eai.3-11-2023.2347957} }- Irena Francina Kelly Kamal
Hermansyah Hermansyah
Desnelli Desnelli
Muharni Muharni
Yosie Andriani
Year: 2024
Sungkai (Peronema canescens Jack) Leaf Methanol Extract for Proliferation of Saccharomyces cerevisiae Yeast and Mice (Mus muculus)
SICBAS
EAI
DOI: 10.4108/eai.3-11-2023.2347957
Abstract
Yeast proliferation is closely associated with cancer cells while leukocytes assay is to immunostimulatory activity. This research was aimed to know of sungkai extract in immunomodulator potency in mice (Mus muculus) and proliferation of Saccharomyces cerevisiae yeast. This research used phytochemistry screening and yeast and mice (Mus musculus) leukocytes assay. It was detected that methanol extract of Sungkai leaf contains terpene, steroid, alkaloid, saponin, and flavone. Yeast growth was observed on OD observation at λ = 600 nm, it was showed that there was no significant difference S.cerevisiae cell growth among in the absence and in the presence of various concentrations of Sungkai leaf extract. Mice leukocytes assay resulted in 15% concentration extract showing the highest content of leukocytes found in the mice tail, 9560 units, while negative control results in the lowest one, 5250 units. It was concluded that sungkai extract has potency as an immunomodulatory agent.
