Proceedings of the 1st International Multi-Disciplinary Conference Theme: Sustainable Development and Smart Planning, IMDC-SDSP 2020, Cyperspace, 28-30 June 2020

Research Article

Phylogenetic relationship among field isolates of mycoplasmas and acholeplasmas in two South Australian dairy herds based on sequencing of a short 16S rRNA gene fragment

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  • @INPROCEEDINGS{10.4108/eai.28-6-2020.2298229,
        author={Abd Al-Bar  Al-Farha},
        title={Phylogenetic relationship among field isolates of mycoplasmas and acholeplasmas in two South Australian dairy herds based on sequencing of a short 16S rRNA gene fragment},
        proceedings={Proceedings of the 1st International Multi-Disciplinary Conference Theme: Sustainable Development and Smart Planning, IMDC-SDSP 2020, Cyperspace, 28-30 June 2020},
        publisher={EAI},
        proceedings_a={IMDC-SDSP},
        year={2020},
        month={9},
        keywords={mycoplasma acholeplasma mastitis phylogeny 16s rrna cattle},
        doi={10.4108/eai.28-6-2020.2298229}
    }
    
  • Abd Al-Bar Al-Farha
    Year: 2020
    Phylogenetic relationship among field isolates of mycoplasmas and acholeplasmas in two South Australian dairy herds based on sequencing of a short 16S rRNA gene fragment
    IMDC-SDSP
    EAI
    DOI: 10.4108/eai.28-6-2020.2298229
Abd Al-Bar Al-Farha1,*
  • 1: Department of Animal Production, Technical Agricultural College, Northern Technical University, Mosul, Iraq. 41002. Email of the corresponding author
*Contact email: dr.abdalbar@ntu.edu.iq

Abstract

Mycoplasma mastitis has been recognized as an emerging disease with a significant impact on the dairy industry. This study aimed to determine the genotypic distribution of some Mycoplasma and Acoleplasma spp isolated from two commercial dairy farms in South Australia based on 16S rRNA sequencing, and compare their evolutionary relationship to some similar isolates from elsewhere. Neighbour-joining phylogeny of 16S rRNA demonstrated the involvement of four different spp. isolated from Farm 1 including: M. bovis, M. bovirhinis, Acholeplasma laidlawii and A. axanthum while M. alkalescens was isolated from Farm 2. Two strains of M. bovis showed similarity to Egyptian, Hungarian and Chinese strains. M. bovirhinis clustered with the Egyptian group. Mycoplasma alkalescens grouped with Swedish and Japanese strains. The Acholeplasma group showed two distinct clusters of A. laidlawii and A. axanthum. Determination of the species/genera involved in mastitis may enhance the molecular epidemiology and in-turn can contribute to controlling strategies of the disease.