Proceedings of the 1st International Multi-Disciplinary Conference Theme: Sustainable Development and Smart Planning, IMDC-SDSP 2020, Cyperspace, 28-30 June 2020

Research Article

Haematological, Biochemical and Histopathological effects of Azathioprine in male guinea pigs

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  • @INPROCEEDINGS{10.4108/eai.28-6-2020.2297943,
        author={Ahlam  Alrekabi and Maitham  Alrekabi and Sawsan  Alhasoon and Ahmad  Nadim},
        title={Haematological, Biochemical and Histopathological  effects of Azathioprine in male guinea pigs},
        proceedings={Proceedings of the 1st International Multi-Disciplinary Conference Theme: Sustainable Development and Smart Planning, IMDC-SDSP 2020, Cyperspace, 28-30 June 2020},
        publisher={EAI},
        proceedings_a={IMDC-SDSP},
        year={2020},
        month={9},
        keywords={azathioprine hematologic biochemical histopathological male pigs},
        doi={10.4108/eai.28-6-2020.2297943}
    }
    
  • Ahlam Alrekabi
    Maitham Alrekabi
    Sawsan Alhasoon
    Ahmad Nadim
    Year: 2020
    Haematological, Biochemical and Histopathological effects of Azathioprine in male guinea pigs
    IMDC-SDSP
    EAI
    DOI: 10.4108/eai.28-6-2020.2297943
Ahlam Alrekabi1,*, Maitham Alrekabi2, Sawsan Alhasoon3, Ahmad Nadim4
  • 1: Department of Physiology, Pharmacology and Biochemistry, College of Veterinary Medicine, University of Basrah, Iraq
  • 2: Department of Clinical Pharmacology, College of Pharmacy, University of Basrah, Iraq
  • 3: Department of Histology and Anatomy, College of Veterinary Medicine, University of Basrah, Iraq
  • 4: College of Veterinary Medicine, University of Basrah, Iraq.
*Contact email: aliabdalnaby19@gmail.com

Abstract

The study was conducted to evaluate the effects of azathioprine on hematologic, biochemical parameters, enzyme activities and histopathological changes on the leg bone, liver, kidney and testes. Two groups of pigs were used. Group 1 was given normal saline and group 2 was given azathioprine (50mg/kg) orally over 4 weeks. The results indicate that azathioprine elicited a significant decrease in blood tests. While, the serum enzymes levels significantly increased in the albumin, urea and creatinine, histopathologically the leg bone exhibited a decrease in hematopoietic cell and an increase in adipocytes in the bone marrow. The liver showed vacuolation of hepatocytes, septal fibrosis and kidney presence vacuolation of cortical areas and dilatation of tubules. In testes, vacuolation and suppression of spermatogenesis in the treated group with azathioprine were noted. It can be concluded that azathioprine induces toxic and hazardous effects on some organs that restrict its use.