Research Article
Evaluation of antioxidant and cytotoxic activities of ethyl acetate extracts of bacterial isolates associated with mangrove soil from the Ngurah Rai mangrove forest, Denpasar, Bali
@INPROCEEDINGS{10.4108/eai.28-10-2022.2328209, author={Anak Agung Gede Indraningrat and Made Dharmesti Wijaya and Dewa Ayu Putri Sri Masyeni and Putu Indah Budi Apsari and Ni Made Defy Janurianti and Devin Daniswara and Ni Luh Putu Puspita Ningrum and Putu Arya Suryanditha and Ni Wayan Widhidewi}, title={Evaluation of antioxidant and cytotoxic activities of ethyl acetate extracts of bacterial isolates associated with mangrove soil from the Ngurah Rai mangrove forest, Denpasar, Bali}, proceedings={Proceedings of the 2nd Warmadewa International Conference on Science, Technology and Humanity, WICSTH 2022, 28-29 October 2022, Denpasar-Bali, Indonesia}, publisher={EAI}, proceedings_a={WICSTH}, year={2023}, month={4}, keywords={bacteria; mangrove; antioxidant; cytotoxicity}, doi={10.4108/eai.28-10-2022.2328209} }- Anak Agung Gede Indraningrat
Made Dharmesti Wijaya
Dewa Ayu Putri Sri Masyeni
Putu Indah Budi Apsari
Ni Made Defy Janurianti
Devin Daniswara
Ni Luh Putu Puspita Ningrum
Putu Arya Suryanditha
Ni Wayan Widhidewi
Year: 2023
Evaluation of antioxidant and cytotoxic activities of ethyl acetate extracts of bacterial isolates associated with mangrove soil from the Ngurah Rai mangrove forest, Denpasar, Bali
WICSTH
EAI
DOI: 10.4108/eai.28-10-2022.2328209
Abstract
Mangrove forest is a natural ecosystem that has been designated as a conservation and tourism area. Bacteria from mangrove communities are known to synthesize various secondary metabolites. However, the bacterial biodiversity associated with soil from mangrove plants, especially those in Bali, has not been explored optimally. This study was designed to analyze the potential of antioxidant activity and cytotoxicity of five bacterial isolates namely Bacillus sp. SA1, Bacillus sp. SA4, Bacillus sp. AM23, Bacillus sp. RM10 and Bacillus sp.RM 18 which were previously isolated from mangrove soil in Ngurah Rai mangrove forest Denpasar Bali. Results showed that ethyl acetate extracts of the five bacterial isolates display weak antioxidant activity with IC50 values > 150 ppm. The cytotoxicity test showed that the etyhyl acetate crude extract of bacterial isolate RM10 inhibted 70% of Artemia salina larvae, followed by crude extract of Bacillus sp. RM18 and Bacillus sp. SA4 isolate extracts which equally inhibited 60% of these larvae. This results indicated the potential of isolate Bacillus sp.RM18, Bacillus sp.RM10 and Bacillus sp. SA4 in producing cytotoxic compounds. GC-MS chemical profiling of ethly acetate Bacillus sp. RM10 showed 11 different compounds. Overall, these findings confirmed the potential bacteria associated with mangrove soil as the source of bioactive compounds.