Research Article
In-vitro α-Glucosidase Inhibitory Activity of Litsea petiolata Hk. f
@INPROCEEDINGS{10.4108/eai.26-10-2020.2311312, author={Neneng Siti Silfi Ambarwati and Berna Elya and Putu Gita Maya Widyaswari Mahayasih and Muhamad Sharul Nizam Awang and Hanita Omar and Islamudin Ahmad and Dwi Atmanto}, title={In-vitro α-Glucosidase Inhibitory Activity of Litsea petiolata Hk. f}, proceedings={Proceedings of the 1st International Conference on Health Science, ICHS 2020, 26-27 October 2020, Jakarta, Indonesia}, publisher={EAI}, proceedings_a={ICHS}, year={2021}, month={8}, keywords={litsea petiolata hk f extract fraction alpha-glucosidase inhibitory}, doi={10.4108/eai.26-10-2020.2311312} }- Neneng Siti Silfi Ambarwati
Berna Elya
Putu Gita Maya Widyaswari Mahayasih
Muhamad Sharul Nizam Awang
Hanita Omar
Islamudin Ahmad
Dwi Atmanto
Year: 2021
In-vitro α-Glucosidase Inhibitory Activity of Litsea petiolata Hk. f
ICHS
EAI
DOI: 10.4108/eai.26-10-2020.2311312
Abstract
Alpha-glucosidase is an enzyme that plays a role in the process of hydrolysis of carbohydrates into glucose so that the enzyme inhibitor can inhibit the process of glucose absorption. One synthetic drug that has a mechanism of action to inhibit the activity of the alpha-glucosidase enzyme is acarbose, however in the long term has side effects, such as digestive system disorders. Therefore, the use of natural medicines as alternative therapies can be considered for use. This study aimed to determine the inhibitory activity of the enzyme alpha-glucosidase from extracts and fractions of Litsea petiolata Hk. f bark. The extraction method used maceration, and fractionation using column chromatography. The results showed that the IC50 values for extract, fraction B, fraction D, and acarbose were 444.17 µg/ml, 682.85 µg/ml, 1683.45 µg/ml, and 110.60 µg/ml, respectively. In conclusion, the activity extract as the alpha-glucosidase was higher than the fractions but lower than acarbose.
