Proceedings of the 1st International Conference on Health Science, ICHS 2020, 26-27 October 2020, Jakarta, Indonesia

Research Article

In-vitro α-Glucosidase Inhibitory Activity of Litsea petiolata Hk. f

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  • @INPROCEEDINGS{10.4108/eai.26-10-2020.2311312,
        author={Neneng Siti Silfi Ambarwati and Berna  Elya and Putu Gita Maya Widyaswari Mahayasih and Muhamad Sharul Nizam Awang and Hanita  Omar and Islamudin  Ahmad and Dwi  Atmanto},
        title={In-vitro α-Glucosidase Inhibitory Activity of Litsea petiolata Hk. f},
        proceedings={Proceedings of the 1st International Conference on Health Science, ICHS 2020, 26-27 October 2020, Jakarta, Indonesia},
        publisher={EAI},
        proceedings_a={ICHS},
        year={2021},
        month={8},
        keywords={litsea petiolata hk f extract fraction alpha-glucosidase inhibitory},
        doi={10.4108/eai.26-10-2020.2311312}
    }
    
  • Neneng Siti Silfi Ambarwati
    Berna Elya
    Putu Gita Maya Widyaswari Mahayasih
    Muhamad Sharul Nizam Awang
    Hanita Omar
    Islamudin Ahmad
    Dwi Atmanto
    Year: 2021
    In-vitro α-Glucosidase Inhibitory Activity of Litsea petiolata Hk. f
    ICHS
    EAI
    DOI: 10.4108/eai.26-10-2020.2311312
Neneng Siti Silfi Ambarwati1, Berna Elya2,*, Putu Gita Maya Widyaswari Mahayasih3, Muhamad Sharul Nizam Awang4, Hanita Omar4, Islamudin Ahmad5, Dwi Atmanto1
  • 1: Department of Cosmetology, Faculty of Engineering, Universitas Negeri Jakarta, Jl. Rawamangun Muka, Jakarta Timur, DKI Jakarta, 13220, Indonesia
  • 2: Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, West Java, Indonesia
  • 3: Department of Pharmacy, Faculty of Health and Sciences, Universitas Esa Unggul, Jakarta, Indonesia
  • 4: Chemistry Division, Centre for Foundation Studies in Science, University of Malaya, Kuala Lumpur, 50603, Malaysia
  • 5: Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Mulawarman, Samarinda, East Kalimantan, Indonesia
*Contact email: berna.elya@gmail.com

Abstract

Alpha-glucosidase is an enzyme that plays a role in the process of hydrolysis of carbohydrates into glucose so that the enzyme inhibitor can inhibit the process of glucose absorption. One synthetic drug that has a mechanism of action to inhibit the activity of the alpha-glucosidase enzyme is acarbose, however in the long term has side effects, such as digestive system disorders. Therefore, the use of natural medicines as alternative therapies can be considered for use. This study aimed to determine the inhibitory activity of the enzyme alpha-glucosidase from extracts and fractions of Litsea petiolata Hk. f bark. The extraction method used maceration, and fractionation using column chromatography. The results showed that the IC50 values for extract, fraction B, fraction D, and acarbose were 444.17 µg/ml, 682.85 µg/ml, 1683.45 µg/ml, and 110.60 µg/ml, respectively. In conclusion, the activity extract as the alpha-glucosidase was higher than the fractions but lower than acarbose.