Proceedings of the International Conference on Sustainable Engineering, Infrastructure and Development, ICO-SEID 2022, 23-24 November 2022, Jakarta, Indonesia

Research Article

Investigation of Methane Production and Microbial Communities of Digested Sewage Sludge on Thermotolerant Anaerobic Digestion

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  • @INPROCEEDINGS{10.4108/eai.23-11-2022.2341542,
        author={Gede Adi Wiguna Sudiartha and Tsuyoshi  Imai},
        title={Investigation of Methane Production and Microbial  Communities of Digested Sewage Sludge on Thermotolerant Anaerobic Digestion},
        proceedings={Proceedings of the International Conference on Sustainable Engineering, Infrastructure and Development, ICO-SEID 2022, 23-24 November 2022, Jakarta, Indonesia},
        publisher={EAI},
        proceedings_a={ICO-SEID},
        year={2023},
        month={12},
        keywords={anaerobic digestion methane production methanogen methanosaeta thermotolerant},
        doi={10.4108/eai.23-11-2022.2341542}
    }
    
  • Gede Adi Wiguna Sudiartha
    Tsuyoshi Imai
    Year: 2023
    Investigation of Methane Production and Microbial Communities of Digested Sewage Sludge on Thermotolerant Anaerobic Digestion
    ICO-SEID
    EAI
    DOI: 10.4108/eai.23-11-2022.2341542
Gede Adi Wiguna Sudiartha1, Tsuyoshi Imai2,*
  • 1: Environmental Engineering Study Program, Faculty of Engineering, Udayana University, Bali 80361, Indonesia
  • 2: Graduate School of Sciences and Technology for Innovation, Yamaguchi University, Yamaguchi 755-8611, Japan
*Contact email: imai@yamaguchi-u.ac.jp

Abstract

The operating temperature of an anaerobic digestion (AD) process regulates CH4 production and microbial communities. This study investigated the CH4 production and microbial communities at the temperature range between mesophilic and thermophilic temperatures, which was defined as the thermotolerant region. Laboratory-scale anaerobic digestion was carried out using 160 ml vials with digested sludge as inoculum. The vials were then incubated under temperature conditions of 42 °C, 45 °C, and 48 °C. A higher temperature condition significantly enhanced the methane production from 0.21, 0.27, to 0.29 L/g COD at 42, 45, and 48 °C respectively. During incubation, the methanogen population was dominated by acetoclastic Methanosaeta, while only a few of hydrogenotrophic methanogens were found. One of the major competitors of Methanosaeta in craving available acetate to produce CH4 is the syntrophic acetate-oxidizing bacteria (SAOB). The high population of SAOB suppressed the growth of Methanosaeta which consequently lower the CH4 production by 12%.