Research Article
Fine Growth on Explants of Manggis Leaf (Garcinia mangostana L.) Planted by In Vitro
@INPROCEEDINGS{10.4108/eai.20-9-2022.2324686, author={Siti Hafizah Al Khairiyah Bintang and Fauziyah Harahap and Melva Silitonga}, title={Fine Growth on Explants of Manggis Leaf (Garcinia mangostana L.) Planted by In Vitro}, proceedings={Proceedings of the 7th Annual International Seminar on Transformative Education and Educational Leadership, AISTEEL 2022, 20 September 2022, Medan, North Sumatera Province, Indonesia}, publisher={EAI}, proceedings_a={AISTEEL}, year={2022}, month={12}, keywords={in vitro zpt vitamin 24 d}, doi={10.4108/eai.20-9-2022.2324686} }- Siti Hafizah Al Khairiyah Bintang
Fauziyah Harahap
Melva Silitonga
Year: 2022
Fine Growth on Explants of Manggis Leaf (Garcinia mangostana L.) Planted by In Vitro
AISTEEL
EAI
DOI: 10.4108/eai.20-9-2022.2324686
Abstract
This study was conducted to determine the effect of growth regulators (ZPT) on callus induction of mangosteen (Garcinia mangostana l. ) from leaf sources grown in vitro. The study was carried out from February 2022 to June 2022. This study used a completely randomized design (CRD) with 2 treatment factors, namely: (a) the first factor concentration of 2,4D consisted of 4 levels, namely: 0 ppm, 1, 5 ppm, 3 ppm and 4.5 ppm. (b) the second factor of vitamin concentration consists of 3 levels, namely: 0 ppm, 1 ppm and 2 ppm. Callus appears first begins with the warping of the explant. Explants that grow are characterized by the appearance of white spots on one or several parts of the explants. The data from the observation of the fastest callus formation in the MediaV2+D3 treatment was the 2nd week. Meanwhile, the time for callus to appear was at the latest in the V2+D1,5 media treatment, which was the 12th week. The height of the callus pile observed at week 12 after planting was carried out by calculating the callus height that grew from all explants. The results of data analysis showed that the V1+D0 media treatment resulted in the highest number of Callus Pile Height 0.60 at 12 WAP observations. The lowest callus heap height yield was obtained from treatment with an average number of callus 0.33 roots.