Research Article
The Formation and Organization of Self-Assembled Monolayer (SAM) for Escherichia coli Detection: A Review
@INPROCEEDINGS{10.4108/eai.20-9-2019.2292104, author={Atqiya Muslihati and Hatijah Basri and Mohd. Zainizan Sahdan and Kusnanto Mukti Wibowo and Nurliyana Rosni and Marlia Morsin}, title={The Formation and Organization of Self-Assembled Monolayer (SAM) for Escherichia coli Detection: A Review}, proceedings={Proceedings of the 2nd International Conference of Science and Technology for the Internet of Things, ICSTI 2019, September 3rd 2019, Yogyakarta, Indonesia}, publisher={EAI}, proceedings_a={ICSTI}, year={2020}, month={3}, keywords={self-assembled monolayers; e coli; biosensor; nanomaterial}, doi={10.4108/eai.20-9-2019.2292104} }- Atqiya Muslihati
Hatijah Basri
Mohd. Zainizan Sahdan
Kusnanto Mukti Wibowo
Nurliyana Rosni
Marlia Morsin
Year: 2020
The Formation and Organization of Self-Assembled Monolayer (SAM) for Escherichia coli Detection: A Review
ICSTI
EAI
DOI: 10.4108/eai.20-9-2019.2292104
Abstract
SAM is known as a tuneable platform through an ultra-thin, biocompatible, and controlled organic film formed by self-assembled from a strong chemisorption of thiols (R-S-H) or its derivatives (R-OH, R-COO, R-COOH, R-NH2, etc.) onto metal or semiconductor substrate. The monolayer is offering hydrophobic-hydrophilic control depending on their terminal group. These flexibilities were functionalized in order to immobilize the biomolecule like DNA/RNA, antibody/antigen, bacteriophages, and aptamers as a selective receptor to capture E. coli selectively as the most dangerous microorganism in environmental water into an array system called biosensor. A number of methods have been developed to characterize the performance of SAM functionalization in immobilizing biomolecule for E. coli rapid detection and real time in optical and electrochemical (amperometric, potentiometric, and impedimetric). Till date, nano-materials and nanotechnologies has significantly contributed as a key role as in basic platform forming the sensor sensitively. Nano materials offer attractive properties like shape dependent physical and unique size, easy synthesize, easy surface functionalization, and enhancement of an electron transfer. This review will discuss the biomolecule immobilization techniques with SAM and the sensing mechanism