Research Article
Plant-Virus Interaction on Orchids Infected Odontoglossum ringspot virus (ORSV) in Bogor Botanical Garden, Indonesia
@INPROCEEDINGS{10.4108/eai.2-5-2019.2284701, author={Mahfut Mahfut and Budi Setiadi Daryono and Ari Indrianto and Soesamto Somowiyarjo}, title={Plant-Virus Interaction on Orchids Infected Odontoglossum ringspot virus (ORSV) in Bogor Botanical Garden, Indonesia}, proceedings={1st International Conference on Science and Technology, ICOST 2019, 2-3 May, Makassar, Indonesia}, publisher={EAI}, proceedings_a={ICOST}, year={2019}, month={6}, keywords={orsv chloroplast coat protein gene rbcl plant-virus interaction bogor botanical garden indonesia}, doi={10.4108/eai.2-5-2019.2284701} }- Mahfut Mahfut
Budi Setiadi Daryono
Ari Indrianto
Soesamto Somowiyarjo
Year: 2019
Plant-Virus Interaction on Orchids Infected Odontoglossum ringspot virus (ORSV) in Bogor Botanical Garden, Indonesia
ICOST
EAI
DOI: 10.4108/eai.2-5-2019.2284701
Abstract
Biotic stress by Odontoglossum ringspot virus (ORSV) infection causes a decrease in the rate of photosynthesis. This study aimed to determine the pattern of plant-virus interactions through analysis of rbcL and coat protein genes. Virus source was collected from Bogor Botanical Garden. Detection of chloroplast and ORSV using Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) and Polymerase Chain Reaction (PCR), respectively. Analyzed change of nucleotide and amino acid sequences using DNASTAR and MEGA Beta programs. Chloroplast DNA and virus RNA amplification managed to get amplicons at ±599 bp and ±474 bp, respectively. Sequence nucleotides analysis showed point mutations with increased synthesis of Ser, Glu, Pro, dan Arg amino acids. This study revealed a change in sequence of nucleotides and amino acids were thought to be adaptations and physiological responses of host-virus to natural environments in Indonesia.
