1st International Conference on Science and Technology, ICOST 2019, 2-3 May, Makassar, Indonesia

Research Article

Real Time PCR Assays for Detection and Quantification of Porcine DNA in Meat Milling Samples

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  • @INPROCEEDINGS{10.4108/eai.2-5-2019.2284634,
        author={M.  Baihaqi and Yuanita  Rachmawati and Saiku  Rokhim and Misbakhul  Munir and Lilik  Hamidah},
        title={Real Time PCR Assays for Detection and Quantification of Porcine DNA in Meat Milling Samples},
        proceedings={1st International Conference on Science and Technology, ICOST 2019, 2-3 May, Makassar, Indonesia},
        publisher={EAI},
        proceedings_a={ICOST},
        year={2019},
        month={6},
        keywords={meat milling porcine dna real time pcr halal certification},
        doi={10.4108/eai.2-5-2019.2284634}
    }
    
  • M. Baihaqi
    Yuanita Rachmawati
    Saiku Rokhim
    Misbakhul Munir
    Lilik Hamidah
    Year: 2019
    Real Time PCR Assays for Detection and Quantification of Porcine DNA in Meat Milling Samples
    ICOST
    EAI
    DOI: 10.4108/eai.2-5-2019.2284634
M. Baihaqi1,*, Yuanita Rachmawati2, Saiku Rokhim2, Misbakhul Munir3, Lilik Hamidah3
  • 1: Department of Arabic, Faculty of Tarbiyah, Universitas Islam Negeri Sunan Ampel Surabaya, Indonesia 60237
  • 2: Department of Biology, Faculty of Science & Technology, Universitas Islam Negeri Sunan Ampel Surabaya, Indonesia 60237
  • 3: Halal Center University, Universitas Islam Negeri Sunan Ampel Surabaya, Indonesia 60237
*Contact email: yuanitarhartono@gmail.com

Abstract

Meat mills in Indonesia are not halal certified. Small traders who do not have a food processor facility will choose the public market. This can be done both by traders who sell halal processed food and processed pork. It is necessary to study the content of meat mills in the public market so that it becomes critical evidence of an institution authorized to issue a policy immediately. This study aims to examine the content of porcine DNA in meat mills in Pasar Surya Kota Surabaya using the Real Time PCR (qPCR) method. QPCR amplification was performed using a porcine probe primer with annealing temperature optimization of 60oC. The amplification curve shows an increase in the curve in the positive control (EPC) with a value of Cq 34.00, while the negative control (NTC) is not amplified. The results of this study show that 2 out of 10 samples experienced an increase in the amplification curve on the FAM indicator, meaning that in the meat grinding sample there was a content of porcine DNA. The two samples are 8 and 9 with Cq values of 38.02 and 39.04, respectively. Market Halal Certification must be done immediately.