Research Article
Phytochemical Analyses of Rosemary (Rosmarinus Officinalis) and Its Effects on the Growth of Propionibacterium Acnes in Mueller Hinton Broth (Mhb) Media
@INPROCEEDINGS{10.4108/eai.2-12-2021.2320281, author={Widhorini Widhorini and Asep Ginanjar Arip and Sofyan H. Nur}, title={Phytochemical Analyses of Rosemary (Rosmarinus Officinalis) and Its Effects on the Growth of Propionibacterium Acnes in Mueller Hinton Broth (Mhb) Media}, proceedings={Proceedings of the 2nd Universitas Kuningan International Conference on System, Engineering, and Technology, UNISET 2021, 2 December 2021, Kuningan, West Java, Indonesia}, publisher={EAI}, proceedings_a={UNISET}, year={2022}, month={8}, keywords={rosmarinus officinalis; phytochemicals propionibacterium acnes; microdilution; minimum inhibitory concentration (mic)}, doi={10.4108/eai.2-12-2021.2320281} }- Widhorini Widhorini
Asep Ginanjar Arip
Sofyan H. Nur
Year: 2022
Phytochemical Analyses of Rosemary (Rosmarinus Officinalis) and Its Effects on the Growth of Propionibacterium Acnes in Mueller Hinton Broth (Mhb) Media
UNISET
EAI
DOI: 10.4108/eai.2-12-2021.2320281
Abstract
Rosemary (Rosmarinus officinalis) is a plant originating from the Mediterranean region and indicated as having antibacterial activity. The purpose of this study was to evaluate the potential phytochemical substances of rosemary in inhibiting the activity of Propionibacterium acnes bacteria using microdilution method and the identification of compounds that have activity as antibacterial use bioautography method. The value of the minimum inhibitory concentration (MIC) against Propionibacterium acne bacteria contained in the ethyl acetate fraction with MIC 512μg/ml. Thin layer chromatography (TLC) monitoring with silica gel stationary phase GF254 and chloroform phase methanol was done in the proportion of 9:1. The antibacterial activity test by bioautography method showed that there were spots on chromatogram of TLC resulting in inhibition zone with Rf value of 0.2, and with the appearance of AlCl3 showed patches of greenish yellow color in a λ366 nm UV lamp on the Rf. Presumably the antibacterial active compound for Propionibacterium acne from the ethyl acetate fraction is a flavonoid group compound. The result of non-parametric statistic test of Kruskal-Wallis followed by Post Hoc test of Mann Whitney obtained conclusion that there was no significant difference between control and ethyl acetate fraction at concentration of 512 acetate fraction at concentration of 512μg/ml
