Proceedings of the 2nd International Conference on Quran and Hadith Studies Information Technology and Media in Conjunction with the 1st International Conference on Islam, Science and Technology, ICONQUHAS & ICONIST, Bandung, October 2-4, 2018, Indonesia

Research Article

Metabolomic Analysis of Dendrophthoe pentandra (L.) Miq. Leaves via UPLC-QToF-MS Coupled with Multivariate Data Analysis using PCA

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  • @INPROCEEDINGS{10.4108/eai.2-10-2018.2295577,
        author={Roihatul  Mutiah and Atina  Yuliandari and Rahmi  Annisa and Burhan  Ma’arif},
        title={Metabolomic Analysis of Dendrophthoe pentandra (L.) Miq. Leaves via UPLC-QToF-MS Coupled with Multivariate Data Analysis using PCA},
        proceedings={Proceedings of the 2nd International Conference on Quran and Hadith Studies Information Technology and Media in Conjunction with the 1st International Conference on Islam, Science and Technology,  ICONQUHAS \& ICONIST, Bandung, October 2-4, 2018, Indonesia},
        publisher={EAI},
        proceedings_a={ICONQUHAS},
        year={2020},
        month={5},
        keywords={metabolomics; dendrophthoe pentandra (l) miq; uplc-qtof-ms/ms; pca (pricipal component analysis)},
        doi={10.4108/eai.2-10-2018.2295577}
    }
    
  • Roihatul Mutiah
    Atina Yuliandari
    Rahmi Annisa
    Burhan Ma’arif
    Year: 2020
    Metabolomic Analysis of Dendrophthoe pentandra (L.) Miq. Leaves via UPLC-QToF-MS Coupled with Multivariate Data Analysis using PCA
    ICONQUHAS
    EAI
    DOI: 10.4108/eai.2-10-2018.2295577
Roihatul Mutiah1,*, Atina Yuliandari1, Rahmi Annisa1, Burhan Ma’arif1
  • 1: UIN Maulana Malik Ibrahim, Departement of Pharmacy, Faculty of Medical and Health Sciences, Malang, Malang, Indonesia
*Contact email: roiha@farmasi.uin-malang.ac.id

Abstract

Mango mistletoe (Dendrophthoe pentandra) is a semi-parasitic plant on a host that is potential as a medicinal plant. Thus, it is necessary to conduct metabolite profiling to determine the compounds contained in mango mistletoe leaves. This research aims to find out the difference of metabolite profile of mango mistletoe leaves obtained from Kediri, East Java; Pekalongan, Central Java; Batin Baru Mountain, Lampung and Tanjung, Selor Hilir in North Kalimantan. Extraction method with ultrasonic aid using 96% ethanol solvent with the ratio of 1:10 [b/v] was used to obtain condensed extract of mango mistletoe leaves. The four extracts from different regions were analyzed using UPLC-QToF-MS/MS, mobile phase of mixture of water/formic acid of 99.9/0.1 [v/v] and acetonitrile/formic acid of 99.9 / 0.1 [v/v] with a gradient elution system and a C18 stationary phase. The results of the analysis were then followed up by a chemometric analysis using PCA employing Minitab 17 software. There are differences in the metabolite among the four regions of extract acquisition. The grouping is based on similar metabolite which occurred in Kediri, East Java and Tanjung Selor Hilir, North Kalimantan. Allegedly, methyldioctylamine; scortechinone F; 3-Cyclohexyl-N- (Ethoxycarbonyl)-L-Alanyl-N-[(4S, 5E, 7R)-7-carbamoyl-9-methyl-5-decen-4-yl]-lysinamide; and Pheophorbide A were biomarker compounds based on the geographic origin.