Metabolomic Compound of Calostropis Gigantea Root Extract and the Inhibition Effect on Fibrosarcoma Growth and Caspase 3 Expression on Balb/c Mus musculus

The root of Calotropis gigantea (thistle) is an Indonesian herb to treat cancer based on empirical and scientific evidences. The study aims to find out the infuence of ethanol extract of thistle root in inhibiting cancer cell growth of Mus musculus fibrosarcoma in vivo and to find out metabolite compounds in the thistle root extract. The effect of cancer cell growth inhibition was tested on Mus musculus inducted with 7.12-dimetilbenzene (α) antrasena (DMBA) and dose treatment of 50, 100 and 150 mg/Kg body weight. The chromatogram was analyzed using masslynx 4.1. The component identification was based on the m/z ratio measured in masslynx and m/z counted in chemdraw. The result of the study showed that the thistle root extract use dose of 50, 100 and 150 mg/Kg body weight were able to increase the weight of fibro sarcoma mice and have a significant influence on caspase-3 expression with cell apoptosis index 24.3 %; 13.3 % and 12.3 % respectively. The result of metabolomic analysis showed 14 compounds found in the root extract. Two major compounds were found out: 4-Chlorobenzenethiol with the area 32.51% and N-[1-(Adamantan-1-yl) ethyl]-2-(1-piperidinyl)-4-quinazolinamine with the area 37.20%. It was indicated responsible for the anticancer activity of thistle root extract.