Proceedings of the 2nd International Conference on Quran and Hadith Studies Information Technology and Media in Conjunction with the 1st International Conference on Islam, Science and Technology, ICONQUHAS & ICONIST, Bandung, October 2-4, 2018, Indonesia

Research Article

Metabolomic Compound of Calostropis Gigantea Root Extract and the Inhibition Effect on Fibrosarcoma Growth and Caspase 3 Expression on Balb/c Mus musculus

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  • @INPROCEEDINGS{10.4108/eai.2-10-2018.2295560,
        author={Roihatul  Mutiah and Maratus  Soliha and Elok Kamilah Hayati and Retno  Susilowati and Rahmi  Annisa},
        title={Metabolomic Compound of Calostropis Gigantea Root Extract and the Inhibition Effect on Fibrosarcoma Growth and Caspase 3 Expression on Balb/c Mus musculus},
        proceedings={Proceedings of the 2nd International Conference on Quran and Hadith Studies Information Technology and Media in Conjunction with the 1st International Conference on Islam, Science and Technology,  ICONQUHAS \& ICONIST, Bandung, October 2-4, 2018, Indonesia},
        publisher={EAI},
        proceedings_a={ICONQUHAS},
        year={2020},
        month={5},
        keywords={calotropis gigantea; caspase-3; fibrosarcoma; metabolomic; uplc-qtof-ms/ms},
        doi={10.4108/eai.2-10-2018.2295560}
    }
    
  • Roihatul Mutiah
    Maratus Soliha
    Elok Kamilah Hayati
    Retno Susilowati
    Rahmi Annisa
    Year: 2020
    Metabolomic Compound of Calostropis Gigantea Root Extract and the Inhibition Effect on Fibrosarcoma Growth and Caspase 3 Expression on Balb/c Mus musculus
    ICONQUHAS
    EAI
    DOI: 10.4108/eai.2-10-2018.2295560
Roihatul Mutiah1,*, Maratus Soliha2, Elok Kamilah Hayati2, Retno Susilowati3, Rahmi Annisa1
  • 1: Pharmacy Departement Faculty of Medical and Health, Universitas Islam Negeri Maulana Malik Ibrahim Malang, Indonesia
  • 2: Chemistry Departement Faculty of Sciences and Technology, Universitas Islam Negeri Maulana Malik Ibrahim Malang, Indonesia
  • 3: Department of Biology Faculty of Sciences and Technology, Universitas Islam Negeri Maulana Malik Ibrahim Malang, Indonesia
*Contact email: roiha@farmasi.uin-malang.ac.id

Abstract

The root of Calotropis gigantea (thistle) is an Indonesian herb to treat cancer based on empirical and scientific evidences. The study aims to find out the infuence of ethanol extract of thistle root in inhibiting cancer cell growth of Mus musculus fibrosarcoma in vivo and to find out metabolite compounds in the thistle root extract. The effect of cancer cell growth inhibition was tested on Mus musculus inducted with 7.12-dimetilbenzene (α) antrasena (DMBA) and dose treatment of 50, 100 and 150 mg/Kg body weight. The chromatogram was analyzed using masslynx 4.1. The component identification was based on the m/z ratio measured in masslynx and m/z counted in chemdraw. The result of the study showed that the thistle root extract use dose of 50, 100 and 150 mg/Kg body weight were able to increase the weight of fibro sarcoma mice and have a significant influence on caspase-3 expression with cell apoptosis index 24.3 %; 13.3 % and 12.3 % respectively. The result of metabolomic analysis showed 14 compounds found in the root extract. Two major compounds were found out: 4-Chlorobenzenethiol with the area 32.51% and N-[1-(Adamantan-1-yl) ethyl]-2-(1-piperidinyl)-4-quinazolinamine with the area 37.20%. It was indicated responsible for the anticancer activity of thistle root extract.