Research Article
Effect of Pegagan and Beluntas Combination on Diestrus Length and Proliferation of Vaginal Epithelium on Rattus norvegicus
@INPROCEEDINGS{10.4108/eai.2-10-2018.2295543, author={Bayyinatul Muchtaromah and Amanatul Mubdah and Bayu Agung Prahardika}, title={Effect of Pegagan and Beluntas Combination on Diestrus Length and Proliferation of Vaginal Epithelium on Rattus norvegicus}, proceedings={Proceedings of the 2nd International Conference on Quran and Hadith Studies Information Technology and Media in Conjunction with the 1st International Conference on Islam, Science and Technology, ICONQUHAS \& ICONIST, Bandung, October 2-4, 2018, Indonesia}, publisher={EAI}, proceedings_a={ICONQUHAS}, year={2020}, month={5}, keywords={pegagan and beluntas leaf extract; diestrus; vaginal maturation index; vaginal epithelium thickness}, doi={10.4108/eai.2-10-2018.2295543} }- Bayyinatul Muchtaromah
Amanatul Mubdah
Bayu Agung Prahardika
Year: 2020
Effect of Pegagan and Beluntas Combination on Diestrus Length and Proliferation of Vaginal Epithelium on Rattus norvegicus
ICONQUHAS
EAI
DOI: 10.4108/eai.2-10-2018.2295543
Abstract
A variety of reproductive health problems often appear to both men and women, so it takes a natural remedy to reduce the usual side effects on the use of chemical drugs. The people of Indonesia have widely known Pegagan (Centella asiatica) and Beluntas (Pluchea indica) as a plant that has benefits as a medicine. The effect of leaf extract of C. Asiatica and P. indica on the female reproductive system can be known by the change of the estrous cycle, especially in diestrus phase and vagina proliferation. This study used 24 Wistar female rats, aged of 2 months, and weighed of 120-150 g. Combination of pegagan and beluntas extract utilizing a ratio of 1: 1. The treatment consisted of control (C) dose of 0 mg/kg bw, the first treatment (T1) 25 mg/kg bw, the second (T2) 50 mg/kg bw, the third (T3) 75 mg/kg bw, the fourth (T4) 125 mg/kg bw and the fifth (T5) is 200 mg/kg bw. Data were analyzed using ANOVA (α 5%) if there were a significant difference then tested continued using DMRT (α 5%). The results showed that combination of leaf extract of pegagan and beluntas significantly influenced the proliferation of vaginal epithelium, indicated by the increase of maturation index and vaginal epithelial thickness, but did not affect to the length of diestrus. The highest vaginal maturation index and vaginal ephitelial thickness value obtained by a T4 dose of 75 mg/kg bw, thus this dose was the most optimal for use as a fertility supplement.