Proceedings of the 8th Annual International Seminar on Transformative Education and Educational Leadership, AISTEEL 2023, 19 September 2023, Medan, North Sumatera Province, Indonesia

Research Article

The Effect of NAA and BAP in Induction of Protocorm Like Bodies (PLB) Cattleya sp. Orchid In Vitro

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  • @INPROCEEDINGS{10.4108/eai.19-9-2023.2340492,
    author={Anita Rasuna Sari Siregar and Fauziyah  Harahap and Idramsa  Idramsa and Emmy Harso Kardhinata and Armaniar  Armaniar},
    title={The Effect of NAA and BAP in Induction of Protocorm Like Bodies (PLB) Cattleya sp. Orchid In Vitro },
    proceedings={Proceedings of the 8th Annual International Seminar on Transformative Education and Educational Leadership, AISTEEL 2023, 19 September 2023, Medan, North Sumatera Province, Indonesia},
    publisher={EAI},
    proceedings_a={AISTEEL},
    year={2023},
    month={12},
    keywords={rotocorm-like bodies cattleya sp in vitro},
    doi={10.4108/eai.19-9-2023.2340492}
}
  • Anita Rasuna Sari Siregar
    Fauziyah Harahap
    Idramsa Idramsa
    Emmy Harso Kardhinata
    Armaniar Armaniar
    Year: 2023
    The Effect of NAA and BAP in Induction of Protocorm Like Bodies (PLB) Cattleya sp. Orchid In Vitro
    AISTEEL
    EAI
    DOI: 10.4108/eai.19-9-2023.2340492
Anita Rasuna Sari Siregar1,*, Fauziyah Harahap2, Idramsa Idramsa2, Emmy Harso Kardhinata3, Armaniar Armaniar3
  • 1: Postgraduate Student of Biology Education Program, Universitas Negeri Medan Jalan Willem Iskandar/Pasar V, Medan 2023, Indonesia
  • 2: Lecture of Biology Education Program, Universitas Negeri Medan Jalan Willem Iskandar/Pasar V, Medan 2023, Indonesia
  • 3: Lecture of Agroecotechnology Study Program, Universitas Panca Budi, Medan, Indonesia
*Contact email: rasuna23@gmail.com

Abstract

Cattleya sp. seeds do not have an endosperm that cause orchid is threatened with extinction. Forming Protocorm-Like Bodies (PLB) that capable of producing large quantities of orchid seeds quickly. The aim was to determine the best interaction of NAA and BAP on growth induction of PLB. The research method used random design with NAA: 0; 0.5; 1; 1.5 ppm and BAP: 0; 0,5; 1 ppm. The data were analyzed with ANAVA and using the colorgrab application. The result showed there is significantly effect of NAA and BAP interaction on the time PLB was formed and there is no significant interaction on the number of PLB. PLB formed the fastest in week 3 with MS + NAA 1.5 ppm + BAP 1 ppm, the highest number of PLB was found in MS + NAA 1.5 ppm + BAP 1 ppm which was 6,67 PLB. The best color of PLB being green

Keywords
rotocorm-like bodies cattleya sp in vitro
Published
2023-12-07
Publisher
EAI
http://dx.doi.org/10.4108/eai.19-9-2023.2340492
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