Biocompatibility Test of Ceramic Materials in Zebrafish Development

The use of dental implants, which be implanted directly into bone is one of the major advances in modern dentistry. One of the prerequisites for the use of bioceramic is biocompatibility, which means being able to interact and connect with living tissue and the physiological environment without causes any adverse effect on the tissue. Zebrafish (Danio rerio) are known as model organisms that have similarities with humans such as the composition of bones, bone cells, and molecular signaling. The purpose of this study was to determine the effect of various bio-ceramic concentrations exposure to the structure of zebrafish bone tissue. The research was conducted in the laboratory of animal structure and development, Faculty of Biology, Gadjah Mada University. Fertilized zebrafish embryos were chosen and exposed to specific concertation of a ceramic material solution on the well plate, as follows 250µg, 500µg, and 750µg for a period of 72 hpf. One-way ANOVA test was used to assess the significance (P<0,05) of the control treatment results and various bio-ceramic concentrations. The results showed no effect of bio-ceramics (CHA-Ag) on survival rate, hatching rate, and heartbeat rate per minute at all exposures. However, some embryos and larvae at the age of 24-72 hpf concentration of bio-ceramics exposure 750μg, indicating were morphological changes such as egg yolk deformation, malformations of the spine, tail, and caudal fin, and stunted body or eye growth.