Research Article
The Optimization of Antibacterial Production Time of Actinomycetes (J4 Isolate) Against Staphylococcus Aureus and Escherichia Coli
@INPROCEEDINGS{10.4108/eai.18-11-2020.2311633, author={Alfian Syarifuddin and Nanik Sulistyani and Ratna Wijayatri}, title={The Optimization of Antibacterial Production Time of Actinomycetes (J4 Isolate) Against Staphylococcus Aureus and Escherichia Coli}, proceedings={Proceedings of the 2nd Borobudur International Symposium on Humanities and Social Sciences, BIS-HSS 2020, 18 November 2020, Magelang, Central Java, Indonesia}, publisher={EAI}, proceedings_a={BIS-HSS}, year={2021}, month={9}, keywords={actinomycetes antibiotic isolate j4 metabolite secondary rizosfer}, doi={10.4108/eai.18-11-2020.2311633} }- Alfian Syarifuddin
Nanik Sulistyani
Ratna Wijayatri
Year: 2021
The Optimization of Antibacterial Production Time of Actinomycetes (J4 Isolate) Against Staphylococcus Aureus and Escherichia Coli
BIS-HSS
EAI
DOI: 10.4108/eai.18-11-2020.2311633
Abstract
There are many cases of antibiotic resistance that cause death in patients infected with bacteria. The increase in cases necessitated the discovery of new antibiotics as an alternative to antibiotics that were already resistant. Bacteria that produce antibiotic compounds are located in the rhizosphere of a plant. One of them is the rhizosphere of the red ginger plant (Zingiber officinale Roscoe var. Rubrum). The bacterial isolate that produces new antibiotic compounds in the rhizosphere of red ginger is Isolate J4. Isolate J4 can produce secondary metabolites in the form of antibiotic compounds after inoculation on Starch Nitrate Broth (SNB) medium and incubated in a few days to obtain optimal antibiotic compounds based on their activity. The purpose of this study was to determine the results of the antibiotic activity of secondary metabolites of isolate J4 in producing antibiotics against Staphylococcus aureus and Escherichia coli bacteria. Antibiotic activity profile was observed by looking at the relationship between the inhibition zone and the incubation time of these isolates. The results showed that isolate J4 obtained optimal antibiotic that inhibited the growth of Staphylococcus aureus bacteria after incubation for ten days with an average inhibition zone diameter of 14.2 mm, whereas for Escherichia coli bacteria after incubation for five days which is 12.3 mm in diameter.