Proceedings of The 5th Annual International Seminar on Trends in Science and Science Education, AISTSSE 2018, 18-19 October 2018, Medan, Indonesia

Research Article

Antibacterial Activity of Endophytic ER-2 Bacterial Isolates from Plant Cotylelobium melanoxylon

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  • @INPROCEEDINGS{10.4108/eai.18-10-2018.2287460,
        author={Idramsa  Idramsa and Endang Sutariningsih Soetarto and Uswatun  Hasanah and Eko  Prasetya},
        title={Antibacterial Activity of Endophytic ER-2 Bacterial Isolates from Plant Cotylelobium melanoxylon},
        proceedings={Proceedings of The 5th Annual International Seminar on Trends in Science and Science Education, AISTSSE 2018, 18-19 October 2018, Medan, Indonesia},
        publisher={EAI},
        proceedings_a={AISTSSE},
        year={2019},
        month={10},
        keywords={cotylelobium melanoxylon bacteria endophytes antibacterial},
        doi={10.4108/eai.18-10-2018.2287460}
    }
    
  • Idramsa Idramsa
    Endang Sutariningsih Soetarto
    Uswatun Hasanah
    Eko Prasetya
    Year: 2019
    Antibacterial Activity of Endophytic ER-2 Bacterial Isolates from Plant Cotylelobium melanoxylon
    AISTSSE
    EAI
    DOI: 10.4108/eai.18-10-2018.2287460
Idramsa Idramsa1,*, Endang Sutariningsih Soetarto2, Uswatun Hasanah1, Eko Prasetya1
  • 1: Department of Biology, Faculty of Mathematics and Natural Sciences the State University of Medan
  • 2: Department of Biology Gadjah Mada University of Yogyakarta
*Contact email: idramsa@unimed.ac.id

Abstract

Endophytic microbes are microorganisms that colonize the parts of plant organs and interact with host plant without causing any symptoms or diseases in the host plant. Endophytic microbial colonization is found in various types of plant organs, namely: roots, stems, leaves and seeds. This study is focused on endophytic bacteria mostly conducted intensively on wild plants or forests in the tropical ecosystems. Various trees or tropical forest plants have potentials as traditional medicine and in cooperation with the bacteria. The C. melanoxylon has been utilized by local community as antidiarrheal medicine because of the content of active antibacterial compounds. The presence of endophytic bacteria in tissues of the stem of C. melanoxylon was observed the bacteria were directly isolated from the tissues by planting pour plate on Tryptic Soya Agar (TSA) medium. The antibacterial activity of endophytic bacteria isolates was examined using a dual cultural assay against Escherichia coli ATCC 35218 and Staphyllococcus aureus ATCC 25923 as a model bacterial. Morphological, physiological, and biochemical characters were identified based on the ability of the isolates to grow. The endophytic bacteria isolate of strain ER-2 was inhibitat the growth of E. coli ATCC 35218 and S. aureus ATCC 25923.