Proceedings of The 5th Annual International Seminar on Trends in Science and Science Education, AISTSSE 2018, 18-19 October 2018, Medan, Indonesia

Research Article

The Development of Sensitive and Selective Potentiometric Biosensor for Urea Assay

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  • @INPROCEEDINGS{10.4108/eai.18-10-2018.2287315,
        author={Manihar  Situmorang and Primsa  Kaban and Rhica  Ayulinova and Wesly  Hutabarat},
        title={The Development of Sensitive and Selective Potentiometric Biosensor for Urea Assay},
        proceedings={Proceedings of The 5th Annual International Seminar on Trends in Science and Science Education, AISTSSE 2018, 18-19 October 2018, Medan, Indonesia},
        publisher={EAI},
        proceedings_a={AISTSSE},
        year={2019},
        month={10},
        keywords={potentiometric biosensor tungsten wire electrode sensitive and selective response urea assay},
        doi={10.4108/eai.18-10-2018.2287315}
    }
    
  • Manihar Situmorang
    Primsa Kaban
    Rhica Ayulinova
    Wesly Hutabarat
    Year: 2019
    The Development of Sensitive and Selective Potentiometric Biosensor for Urea Assay
    AISTSSE
    EAI
    DOI: 10.4108/eai.18-10-2018.2287315
Manihar Situmorang1,*, Primsa Kaban1, Rhica Ayulinova1, Wesly Hutabarat1
  • 1: Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Negeri Medan
*Contact email: msitumorang@unimed.ac.id

Abstract

The development of the biosensor as a step to obtain analytical instrument for urea is explained in this study. The aim of this research is to obtain sensitive and selective potentiometric biosensor for urea assay in clinical sample. The study was conducted through preparation of an enzyme electrode with immobilisation of urease onto the tungsten wire by using matrix polymer, followed by interfacing the sensing device vs Ag/AgCl with voltmeter that was connected with Powerlab for data aquisition. The presence of urease in the sensing device is catalytically converted the urea to ammonium ion and bicarbonate which results in the potential change in electrochemical cell. The wide linearity range for urea standard solution was obtained (0.1 - 10 mM urea) with slope 42.7 mV per-decade urea concentration (r2 = 0.993). The urea biosensor gives sensitive response to urea where the detection a limit was 0.1 mM urea. A very fast analytical response was achieved that was only taken four minute per-sample. The potentiometric urea biosensor developed in this study is promising since the instrumentation component is relatively inexpensive and can be modified to an portable mini biosensor.