Proceedings of the 1st EAI International Conference on Medical And Health Research, ICoMHER November 13-14th 2018, Padang, West Sumatera, Indonesia

Research Article

The Effect of Mesenchymal Stem Cells on IL-1β and IL-6 in Synoviocytes-Derived Osteoarthritis Grade IV

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  • @INPROCEEDINGS{10.4108/eai.13-11-2018.2283792,
        author={Rizki  Rahmadian and Menkher  Manjas and Roza  Silvia and Hirowati  Ali},
        title={The Effect of Mesenchymal Stem Cells on IL-1β and IL-6 in Synoviocytes-Derived Osteoarthritis Grade IV},
        proceedings={Proceedings of the 1st EAI International Conference on Medical And Health Research, ICoMHER November 13-14th 2018, Padang, West Sumatera, Indonesia},
        publisher={EAI},
        proceedings_a={ICOMHER},
        year={2019},
        month={5},
        keywords={il-6 il-1β mesenchymal stem cells osteoarthritis},
        doi={10.4108/eai.13-11-2018.2283792}
    }
    
  • Rizki Rahmadian
    Menkher Manjas
    Roza Silvia
    Hirowati Ali
    Year: 2019
    The Effect of Mesenchymal Stem Cells on IL-1β and IL-6 in Synoviocytes-Derived Osteoarthritis Grade IV
    ICOMHER
    EAI
    DOI: 10.4108/eai.13-11-2018.2283792
Rizki Rahmadian1,*, Menkher Manjas2, Roza Silvia3, Hirowati Ali4
  • 1: Department of Orthopedic Surgery, Faculty of Medicine, Universitas Andalas M.Djamil Hospital
  • 2: Graduate School of Biomedical Sciences, Andalas University
  • 3: Department of Histology, Faculty of Medicine, Andalas University
  • 4: Department of Biochemistry, Faculty of Medicine, Andalas University
*Contact email: rizki_md@yahoo.com

Abstract

Osteoarthritis is a degenerative joint disease involving activated synovial tissue due to inflammation. Cytokines seem to enhance the matrix components of lead to destruction of cartilage. Mesenchymal stem cells (MSCs) are widely used for cell-based nowadays approaches, thus activated cytokines, IL-1β andIL-6 as a result of synovial inflammation that are left to be investigated.This is an in vitro study of IV-IL and IL-6 on IV synoviocytes-derived osteoarthritis treated with MSCs. Cells were isolated using explants culture method and cultured in DMEM with 10% fetal bovine serum, 1% penicillin streptomycin, and amphotericin B. Cells were incubated at 370C and 5% CO2. After third passages, cells were co-cultured with MSCs. Expression of IL-1β and IL-6 are observed using realtime polymerase chain reaction (PCR) after 24 hours and 48 hours. The expression of IL-1β is marked up after 24 hours of co-cultured with MSCs (57.72 ± 18.37) compared to control and shows a tendency to downgrade after 48 hours of event, statictically not significant. However, IL-6 showed a higher expression in synoviocytes after 24 hours of co-cultured with MSCs, and was significantly down regulated after 48 hours. Both IL-1 β and IL-6 showed higher expressions on 24 hours co-culturing synoviocytes with MSCs. Not IL-1β, expression of IL-6 is significantly decreased after 48 hours MSC treatment, implying that MSV grade IV syndicarthritis-derived osteoarthritis.