Proceedings of the 1st EAI International Conference on Medical And Health Research, ICoMHER November 13-14th 2018, Padang, West Sumatera, Indonesia

Research Article

The Effect of Dadiah Feeding of Breastfeeding Women with Normal Flora of Digestive Tract

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  • @INPROCEEDINGS{10.4108/eai.13-11-2018.2283649,
        author={Netti  Suharti and Linosefa  Linosefa and Asih  Kumala and Eva  Chundrayetti and Andani Eka Putra},
        title={The Effect of Dadiah Feeding of Breastfeeding Women with Normal Flora of Digestive Tract},
        proceedings={Proceedings of the 1st EAI International Conference on Medical And Health Research, ICoMHER November 13-14th 2018, Padang, West Sumatera, Indonesia},
        publisher={EAI},
        proceedings_a={ICOMHER},
        year={2019},
        month={5},
        keywords={dadiah breastfeeding mothers feces normal flora l plantarum},
        doi={10.4108/eai.13-11-2018.2283649}
    }
    
  • Netti Suharti
    Linosefa Linosefa
    Asih Kumala
    Eva Chundrayetti
    Andani Eka Putra
    Year: 2019
    The Effect of Dadiah Feeding of Breastfeeding Women with Normal Flora of Digestive Tract
    ICOMHER
    EAI
    DOI: 10.4108/eai.13-11-2018.2283649
Netti Suharti1,*, Linosefa Linosefa1, Asih Kumala2, Eva Chundrayetti3, Andani Eka Putra1
  • 1: Department of Microbiology, Faculty of Medicine, Universitas Andalas Padang, Indonesia
  • 2: Biomedical Science Graduate Program, Faculty of Medicine, Universitas Andalas Padang, Indonesia
  • 3: Department of Pediatrics, Faculty of Medicine, Universitas Andalas Padang, Indonesia
*Contact email: nettisuharti@med.unand.ac.id

Abstract

Dadiah is a fermented buffalo milk product containing many potential lactic acid bacteria as probiotics. Lactic acid bacteria and their derivative products can prevent the onset of various diseases such as spur health and heart work, good food to increase stamina and endurance, prevent colon cancer and improve intestinal microflora. The objective of this research is to see the effect of giving dadiah in the form of ice cream to the growth of L. Interventions were performed for 1 month, and analysis was performed on the infant's feces. DNA isolated from feces and L. Plantarum concentrations were analyzed using real-time PCR. The standard curve is designed to get the concentration of bacteria appropriately. Data analysis using SPSS version 20.0, numerical data were analyzed by using independent T-test and dependent T-test. P-value of <0.05 was considered statistically significant. The results showed that the mean intestinal microflora of the infant before the dadiah ice cream was given was 4.2 x 107 CFU / gram and after the dadiah ice cream was 8.6 x 108 CFU / g (p <0.05) while in the control group, the mean L. Plantarum intestinal infants before administered whey ice cream is 2.6 x 107 CFU / gram and thereafter 1.3 x 107 CFU / g (p> 0.05). Based on the results of this study it can be concluded that there is an increase in L.plantarum concentration after the provision of dadiah ice cream in breastfeeding mothers