Proceedings of the 1st EAI International Conference on Medical And Health Research, ICoMHER November 13-14th 2018, Padang, West Sumatera, Indonesia

Research Article

The Association of Cyp1a1 Gene Polymorphisms with Cyp1a1 Enzymes, P53 Protein, and Vascular Endothelial Growth Factor Levels in Pterygium

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  • @INPROCEEDINGS{10.4108/eai.13-11-2018.2283641,
        author={Hendriati  Hendriati and Yanwirasti  Yanwirasti and Tjahjono Darminto Gondhowiardjo and Djong Hon Tjong},
        title={The Association of Cyp1a1 Gene Polymorphisms with Cyp1a1 Enzymes, P53 Protein, and Vascular Endothelial Growth Factor Levels in Pterygium},
        proceedings={Proceedings of the 1st EAI International Conference on Medical And Health Research, ICoMHER November 13-14th 2018, Padang, West Sumatera, Indonesia},
        publisher={EAI},
        proceedings_a={ICOMHER},
        year={2019},
        month={5},
        keywords={inflammation polymorphism genetic pterygium},
        doi={10.4108/eai.13-11-2018.2283641}
    }
    
  • Hendriati Hendriati
    Yanwirasti Yanwirasti
    Tjahjono Darminto Gondhowiardjo
    Djong Hon Tjong
    Year: 2019
    The Association of Cyp1a1 Gene Polymorphisms with Cyp1a1 Enzymes, P53 Protein, and Vascular Endothelial Growth Factor Levels in Pterygium
    ICOMHER
    EAI
    DOI: 10.4108/eai.13-11-2018.2283641
Hendriati Hendriati1,*, Yanwirasti Yanwirasti2, Tjahjono Darminto Gondhowiardjo3, Djong Hon Tjong4
  • 1: Ophthalmology Departement of Medical Faculty, Universitas Andalas, Padang –Indonesia
  • 2: Biomedical Laboratory of Universitas Andalas, Indonesia
  • 3: Ophthalmology Departement of Medical Faculty - University of Indonesia, Jakarta –Indonesia
  • 4: Biological Faculty of Universitas Andalas, Padang, Indonesia
*Contact email: hendriati@med.unand.ac.id

Abstract

CYP1A1 gene, which has role in carcinogenic metabolisms, is also detected in pterygium tissue. We aim to determine the association between CYP1A1m1 (rs4646903) polymorphisms with the level of CYP1A1 enzymes, p53 protein, and vascular endothelial growth factor (VEGF) in patients with inflammatory and non-inflammatory pterygium. DNA isolation was performed from a blood sample of 70 pterygium patients consisting of 35 inflammatory and 35 noninflammatory pterygia. Rs 4646903 SNP Genotyping T> C (m1) in the CYP1A1 gene was performed using restriction fragment length polymorphisms-PCR (RFLP-PCR). PCR products confirmed and sent to Macrogen, South Korea for sequencing. Polymorphism results are characterized as wild-type (TT), mutant homozygote (TC), and mutant heterozygote (CC). CYP1A1 gene polymorphisms consist of mutant heterozygote (TC), mutant homozygote (CC) and wild-type (TT). In both groups, the heterozygote mutant was higher than the wild-type and mutant homozygote. The CYP1A1 enzyme level was higher in inflammatory pterygium, P53 protein levels were higher in the non-inflammatory group, and VEGF levels were higher in the inflammatory group. CYP1A1 polymorphisms were not associated with CYP1A1 enzyme levels, p53 protein levels, and VEGF in both groups. CYP1A1 gene polymorphism has not been shown to be associated with levels of CYP1A1 enzymes, p53, and VEGF in both pterygium groups