Proceedings of the 1st EAI International Conference on Medical And Health Research, ICoMHER November 13-14th 2018, Padang, West Sumatera, Indonesia

Research Article

Genetic variation and genetic structure of Aedes aegypti population Dengue Hemorrhagic Fever (DHF) vector in West Sumatera

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  • @INPROCEEDINGS{10.4108/eai.13-11-2018.2283638,
        author={Hasmiwati  Hasmiwati and Supargiyono  Supargiyono},
        title={Genetic variation and genetic structure of Aedes aegypti population Dengue Hemorrhagic Fever (DHF) vector in West Sumatera},
        proceedings={Proceedings of the 1st EAI International Conference on Medical And Health Research, ICoMHER November 13-14th 2018, Padang, West Sumatera, Indonesia},
        publisher={EAI},
        proceedings_a={ICOMHER},
        year={2019},
        month={5},
        keywords={aedes aegypti dengue hemorrhagic fever genetic structure of the population genetic variation},
        doi={10.4108/eai.13-11-2018.2283638}
    }
    
  • Hasmiwati Hasmiwati
    Supargiyono Supargiyono
    Year: 2019
    Genetic variation and genetic structure of Aedes aegypti population Dengue Hemorrhagic Fever (DHF) vector in West Sumatera
    ICOMHER
    EAI
    DOI: 10.4108/eai.13-11-2018.2283638
Hasmiwati Hasmiwati1,*, Supargiyono Supargiyono2
  • 1: Department of Parasitology, Faculty of Medicine, Andalas University, Padang
  • 2: Department of Parasitology, Faculty of Medicine, Gajah Mada University, Yogyakarta
*Contact email: hasmiwati65@med.unand.ac.id

Abstract

Aedes aegypti is the main vector of dengue virus in the tropics and subtropics. Prevention of dengue depends on vector control due to the vaccine is still in development, understanding the survival of the species can be determined by tracking the genetic variations among individuals and determine the genetic structure of Aedes aegypti population. The purpose of this study was to analyze genetic variation and genetic structure of populations of Ae. aegypti in four dengue-endemic region in Sumatra Barat. All collected sample was performed DNA extraction to obtain DNA genomic of Ae. aegypti. DNA was amplified by using microsatellite primers resulted from previous study. Homozygous and heterozygous alleles were detected by using PAGE. Genetic variation and genetic differentiation were high in four dengue-endemic regions in West Sumatra. He scores ranged from 0.7886 to 08,404, I score were 1.5215 to 1.7263, FST score was 0.1205, and Nm was 1.8240. Genetic variation and gene flow of Ae. aegypti among populations, so that is easy for mosquito to adapt. There were two groups of Ae. aegypti resulted based on UPGMA analysis, and there was no correlation between genetic distance and geographical distance. Genetic variation of Ae. aegypti in some endemic areas of dengue fever in West Sumatra was high with moderate genetic differentiation. There were two groups of population based on genetic structure. There was no correlation between genetic distance and geographic distance