Research Article
Correlation of Inflammation Mediator Profile Expression Between Tissues and Mucosal in Chronic Rhinosinusitis with Nasal Polyp
@INPROCEEDINGS{10.4108/eai.13-11-2018.2283554, author={Effy Huriyati}, title={Correlation of Inflammation Mediator Profile Expression Between Tissues and Mucosal in Chronic Rhinosinusitis with Nasal Polyp}, proceedings={Proceedings of the 1st EAI International Conference on Medical And Health Research, ICoMHER November 13-14th 2018, Padang, West Sumatera, Indonesia}, publisher={EAI}, proceedings_a={ICOMHER}, year={2019}, month={5}, keywords={chronic rhinosinusitis with nasal polyps interleukin-5 interleukin-8 interleukin-17a}, doi={10.4108/eai.13-11-2018.2283554} }- Effy Huriyati
Year: 2019
Correlation of Inflammation Mediator Profile Expression Between Tissues and Mucosal in Chronic Rhinosinusitis with Nasal Polyp
ICOMHER
EAI
DOI: 10.4108/eai.13-11-2018.2283554
Abstract
There are differences inflammatory patterns in Chronic Rhinosinusitis with Nasal Polyp (CRSwNP) between Caucasians and Asians. One of the most convenient and non-invasive examination tools to find out the inflammatory pattern of CRSwNP is such as mucosal brushing of the polyp. This research investigated the correlation of expression IL-5, IL-8, and IL-17A between the tissue and mucosal polyp.A cross-sectional comparative study involving 15 samples of mucosal brushing and 15 of polyp tissues were performed. We measured IL-5, IL-8, and IL-17A level of mucosal brushing with the Enzyme-Linked Immunosorbent Assay (ELISA) and polyp tissues with immunohistochemistry (IHC) examination. The mean expression of IL-5, IL-8, and IL-17A by IHC was 62.07% (26.294), 87.33% (16.969), 88.20% (16.781) and ELISA was 0.86 (0.13), 1930.60 (879.83), 25.60 (16.969). There was no significant relationship between tissue (IHC) and mucosa (ELISA) (p> 0.05), but in IL-5 expression there was a moderate positive correlation (0.41<r<0.60).The moderate positive correlation between mucosa and tissue polyp concludes that simple tests such as mucosal brushing can be used to see IL-5 expression in CRSwNP. However, IHC and ELISA tests on IL-8 and IL-17A had negative correlation because there is a variability inflammatory expression between mucosal dan tissues. Mucosal brushing can be used as a simple and non-invasive examination to see IL-5 expression in CRSwNP