THE INFLUENCE OF SALIVARY GLAND EXTRACT (SGE) OF Anopheles sundaicus TO INTERLEUKIN 12 LEVEL OF THE WHITE MOUSE INFECTED WITH Plamodium berghei

Malaria is an infectous disease which is still a major public health problem in the world. A main problem of management of malaria to date is a difficulty finding a effective vaccine providing the protection to people. The aim to study is to know influence of salivary gland extract (SGE) of Anopheles sundaicus to IL-12 level in the white mouse Mus musculus Balb/C after infected with Plasmodium berghei. This research is the laboratory experimental studies with the post test control group design only using animal model as the investigation object. The samples is divided into the control group (mice immunized with adjuvant+PBS, ratio 1:1); The pellets extract group (the mice immunized by pellet of SGE of An. sundaicus + adjuvant, ratio 1:1); the supernathan extract group (the mice immunized by yhe extract of gland supernathan of An. sundaicus + adjuvant, ratio 1:1). Blood sampling of mice is coducted through reorbital plexus a day before the first immuniztation (PP1), a week post third immunization (PP2), 24 hours post infection with P. berghei, and 6 days post infection with P. berghei. Subsequently, from blood plasm obtained was examined IL-12 level by ELISA method and read at 450 nm. The result show pellet gland extract of An. sundaicus provide markedly effect against IL-12. This suggest that mosquito salivary gland of An. sundaicus from Pellet Extract group is able serve as immuno-modulator factors by exciting cellular immune resposes toward Th1