Research Article
Electrochemical Label-Free Immunosensor for The Detection of Epithelial Sodium Channels Using Gold Modified Screen-Printed Carbon Electrode
@INPROCEEDINGS{10.4108/eai.11-7-2019.2298070, author={Yeni Wahyuni Hartati and Nadya Putri Satriana and Shabarni Gaffar and Jacob Yan Mulyana and Santhy Wyantuti and Yulia Sofiatin}, title={Electrochemical Label-Free Immunosensor for The Detection of Epithelial Sodium Channels Using Gold Modified Screen-Printed Carbon Electrode}, proceedings={Proceedings of the 1st International Conference on Islam, Science and Technology, ICONISTECH 2019, 11-12 July 2019, Bandung, Indonesia.}, publisher={EAI}, proceedings_a={ICONISTECH}, year={2021}, month={1}, keywords={anti-enac electrochemical immunosensor epithelial sodium channels (enac) screen printed carbon electrode (spce)}, doi={10.4108/eai.11-7-2019.2298070} }- Yeni Wahyuni Hartati
Nadya Putri Satriana
Shabarni Gaffar
Jacob Yan Mulyana
Santhy Wyantuti
Yulia Sofiatin
Year: 2021
Electrochemical Label-Free Immunosensor for The Detection of Epithelial Sodium Channels Using Gold Modified Screen-Printed Carbon Electrode
ICONISTECH
EAI
DOI: 10.4108/eai.11-7-2019.2298070
Abstract
The epithelial sodium channel (ENaC) is a protein used in maintaining sodium levels and related to hypertension. ENaC protein is abundant in lung, gut, and kidney. An electrochemical immunosensor has developed to detect the level of ENaC in the urine. The electrode used was screen printed carbon electrode-gold (SPCE-Au) via HAuCl4 electrodeposition. Anti-ENaC was immobilized onto the SPCE-Au with 3-mercaptopropinonic (MPA) linker between gold and anti-ENaC. Various concentration of standard ENaC was used to determine the optimum experiment condition and analytical parameters. The interaction of Anti-ENaC that immobilized on the electrode surface with ENaC was measured as a ferric cyanide response by differential pulse voltammetry. The SPCE-Au has increased the ferric cyanide peak current by about 482% compare to SPCE bare. Immobilization of the Anti-ENaC decreases the peak current of about 53%. The optimum conditions of the experiment were obtained; 0.01 M of MPA for 20 min incubation time, and 0.5 µg/mL of anti-EnaC concentration. The sensitivity of the immunosensor was 10.571, the limit of detection of 0.037 ng/mL, and the limit of quantification 0.123 ng/mL for ENaC concentration range from 0.1 to 1.5 ng/mL. Furthermore, the proposed immunosensor can be used to measure the concentration of ENaC as a biomarker of hypertension in clinical urine samples.