Proceedings of the 1st International Conference on Islam, Science and Technology, ICONISTECH 2019, 11-12 July 2019, Bandung, Indonesia.

Research Article

Characterization of A Raw Starch Degrading Alpha Amylase From The Rhizosphere Soil Citatah Karst Region Bacillus sp. K2Br5

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  • @INPROCEEDINGS{10.4108/eai.11-7-2019.2298048,
    author={Tina Dewi Rosahdi and Tia Bestiana Nur Azizah and Fitriani Nurul Hidayati and Rija Lailatus Syifa and Risma  Khoerunnisa and Galih  Lenggana and Anggita Rahmi Hafsari},
    title={Characterization of A Raw Starch Degrading Alpha Amylase From The Rhizosphere Soil Citatah Karst Region Bacillus sp. K2Br5},
    proceedings={Proceedings of the 1st International Conference on Islam, Science and Technology, ICONISTECH 2019, 11-12 July 2019, Bandung, Indonesia.},
    publisher={EAI},
    proceedings_a={ICONISTECH},
    year={2021},
    month={1},
    keywords={α-amylase bacillus sp k2br5 raw starch},
    doi={10.4108/eai.11-7-2019.2298048}
}
  • Tina Dewi Rosahdi
    Tia Bestiana Nur Azizah
    Fitriani Nurul Hidayati
    Rija Lailatus Syifa
    Risma Khoerunnisa
    Galih Lenggana
    Anggita Rahmi Hafsari
    Year: 2021
    Characterization of A Raw Starch Degrading Alpha Amylase From The Rhizosphere Soil Citatah Karst Region Bacillus sp. K2Br5
    ICONISTECH
    EAI
    DOI: 10.4108/eai.11-7-2019.2298048
Tina Dewi Rosahdi1,*, Tia Bestiana Nur Azizah1, Fitriani Nurul Hidayati1, Rija Lailatus Syifa1, Risma Khoerunnisa1, Galih Lenggana1, Anggita Rahmi Hafsari2
  • 1: Chemistry of Department, Faculty of Science and Technology, UIN Sunan Gunung Djati Bandung
  • 2: Biology of Department, Faculty of Science and Technology, UIN Sunan Gunung Djati Bandung
*Contact email: tina_dr@uinsgd.ac.id

Abstract

α-Amylase from Bacillus sp. K2Br5, a bacterium isolated from the Rhizosphere soil, Citatah Karst region, is partially purified by 60% ammonium sulfate precipitation. It displays optimum activity at pH 6.0 and temperature 40 ºC. The presence of Zn2+ and Ca2+ metal ions increased the activity of α-amylase, whereas Cu2+, Na+ and Mn+ metal ions and EDTA as chelating agent decreased the activity of α-amylase. Futhermore, the addition of acarbose as inhibitor and NaCl decreased enzyme activity. α-Amylase from Bacillus sp. K2Br5 showed ability to degrade wheat, rice, cassava, potato and corn starches with adsorption percentage in the range of 29-69%. Wheat has the highest degree of hydrolysis followed by corn, rice, cassava and potato, consecutively at relative low temperatures. Meanwhile, rice has the highest adsorption percentage. The results show α-amylase from Bacillus sp. K2Br5 is a good candidate for low temperature starch processing.

Keywords
α-amylase bacillus sp k2br5 raw starch
Published
2021-01-12
Publisher
EAI
http://dx.doi.org/10.4108/eai.11-7-2019.2298048
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