Research Article
Electrochemical Biosensor for Mitochondrial DNA of Sus Scrofa Detection Using A Cerium-Modified Screen Printed Carbon Electrode
@INPROCEEDINGS{10.4108/eai.11-7-2019.2297724, author={Maulida Fajriyah and Yusuf Rohmatulloh and Shabarni Gaffar and Anni Angraen and Yeni Wahyuni Hartat}, title={Electrochemical Biosensor for Mitochondrial DNA of Sus Scrofa Detection Using A Cerium-Modified Screen Printed Carbon Electrode}, proceedings={Proceedings of the 1st International Conference on Islam, Science and Technology, ICONISTECH 2019, 11-12 July 2019, Bandung, Indonesia.}, publisher={EAI}, proceedings_a={ICONISTECH}, year={2021}, month={1}, keywords={sus scrofa spce-ce electrochemical dna biosensor differential pulse voltammetry}, doi={10.4108/eai.11-7-2019.2297724} }- Maulida Fajriyah
Yusuf Rohmatulloh
Shabarni Gaffar
Anni Angraen
Yeni Wahyuni Hartat
Year: 2021
Electrochemical Biosensor for Mitochondrial DNA of Sus Scrofa Detection Using A Cerium-Modified Screen Printed Carbon Electrode
ICONISTECH
EAI
DOI: 10.4108/eai.11-7-2019.2297724
Abstract
Cerium is one of the rare elements with high abundance and has been widely used in sensor applications. In this study, electrochemical DNA biosensor has been developed to detect mitochondrial DNA (mtDNA) of Sus scrofa using a screen-printed carbon electrode-cerium (SPCE-Ce). The surface of the SPCE electrode before and after modification with cerium was characterized using scanning electron microscopy (SEM) and cyclic voltammetry. The design of the pig nucleotide sequence as probe DNA was determined using NCBI blast and t-coffee programs. The cyclic voltammograms showed a redox current peak rise from the electroactive ferric cyanide species at SPCE-Ce. The probe sequence is 5'-TATTIATACCAATCACTAIC-3' has an identity of 100% with cytochrome b (Cyt b) mtDNA of Sus scrofa. Hybridization probe-target DNA characterized using voltammetry produce peak oxidation of guanine range potential to be at 1.20 V to 1.22 V. The optimum experimental conditions were carried out at 30.0 µg/mL for probe concentration, 20 minutes for immobilization time, and 20 minutes for hybridization time. The linear equation of the target concentration against was I = 0.0303[target]+3.947 for the target DNA concentration range 5.0 to 30.0 µg/mL. Determination of analytic parameters showed that the values of sensitivity, LoD, LoQ, and precision were 0.0303 µg/mL, 1.44 µg/mL, 4.81 µg/mL, and 98.99%, respectively. This biosensor can be used to determine pig mtDNA concentrations in real samples.
