Research Article
The Effect of Various Sterilization Techniques to the Growth of Fingerroot Explants (Boesenbergia Pandurata Roxb)
@INPROCEEDINGS{10.4108/eai.11-7-2019.2297684, author={Epin Pinasti and Liberty Chaidir and Dikayani Dikayani and Adjat Sudrajat}, title={The Effect of Various Sterilization Techniques to the Growth of Fingerroot Explants (Boesenbergia Pandurata Roxb)}, proceedings={Proceedings of the 1st International Conference on Islam, Science and Technology, ICONISTECH 2019, 11-12 July 2019, Bandung, Indonesia.}, publisher={EAI}, proceedings_a={ICONISTECH}, year={2021}, month={1}, keywords={fingerroot shoot sterilization}, doi={10.4108/eai.11-7-2019.2297684} }- Epin Pinasti
Liberty Chaidir
Dikayani Dikayani
Adjat Sudrajat
Year: 2021
The Effect of Various Sterilization Techniques to the Growth of Fingerroot Explants (Boesenbergia Pandurata Roxb)
ICONISTECH
EAI
DOI: 10.4108/eai.11-7-2019.2297684
Abstract
Fingerroot (Boesenbergia pandurata Roxb.) is included to the Zingiberaceae family which has many benefits. Fingerroot crop production has descreaded by 24.46% from 2014-2016. This is because the provision of plant seeds tends to decrease, this is because nurseries rely solely on the harvest, so propagation can be done through tissue culture. This research aims to determine the most effective sterilization techniques for growth fingerroot explants. This research has conducted at Tissue Culture Laboratory of Horticulture Seedlings and Various Plants Pasir Banteng Sumedang, West Java, from March 2018 to February 2019. The method used is descriptive method. The results showed that the use sterilization material was detergent 1 g 100 mL-1 (immersion time for 5 minutes), antibacterial solution 3 drops 100 mL-1 (immersion time for 60 minutes), insecticide 11 g L-1 (immersion time for 60 minutes), fungicide 10 g L-1 (immersion time for 120 minutes), bactericidal 10 g L-1 (immersion time 60 for minutes), alcohol 70% (immersion time for 5 minutes), HgCl 0.2% (immersion time for 7 minutes) and NaClO (immersion time for 10 minutes) gave the best results with the number of live explants as much as 14.8%.