International Conference on Environmental Awareness for Sustainable Development in conjunction with International Conference on Challenge and Opportunities Sustainable Environmental Development, ICEASD & ICCOSED 2019, 1-2 April 2019, Kendari, Indonesia

Research Article

Fermentation Kinetics of Isolate Lactic Acid Bacteria Probiotic BR 12 and BR 17 were Isolated from Broiler Chicken Meat

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  • @INPROCEEDINGS{10.4108/eai.1-4-2019.2287183,
        author={Roisu Eny Mudawaroch and Setiyono  Setiyono and Lies Mira Yusiati and Edi  Suryanto},
        title={Fermentation Kinetics of Isolate Lactic Acid Bacteria  Probiotic BR 12 and BR 17 were Isolated from Broiler Chicken Meat},
        proceedings={International Conference on Environmental Awareness for Sustainable Development in conjunction with International Conference on Challenge and Opportunities Sustainable Environmental Development, ICEASD \& ICCOSED 2019, 1-2 April 2019, Kendari, Indonesia},
        publisher={EAI},
        proceedings_a={ICEASD\&ICCOSED},
        year={2019},
        month={9},
        keywords={fermentation kinetics lactobacillus fermentum br 17 broiler chicken meat},
        doi={10.4108/eai.1-4-2019.2287183}
    }
    
  • Roisu Eny Mudawaroch
    Setiyono Setiyono
    Lies Mira Yusiati
    Edi Suryanto
    Year: 2019
    Fermentation Kinetics of Isolate Lactic Acid Bacteria Probiotic BR 12 and BR 17 were Isolated from Broiler Chicken Meat
    ICEASD&ICCOSED
    EAI
    DOI: 10.4108/eai.1-4-2019.2287183
Roisu Eny Mudawaroch1,*, Setiyono Setiyono2, Lies Mira Yusiati2, Edi Suryanto2
  • 1: Universitas Muhammadiyah Purworejo, Purworejo, Indonesia
  • 2: Universitas Gadjah Mada Fakultas Perternakan,Yogyakarta, Indonesia
*Contact email: roisueny@umpwr.ac.id

Abstract

The purpose of this study was to determine the Lineweaver-Burk equation, maximum speed (μmax) and maximum specific / per hour growth rate Ks (constant saturation) of probiotic lactic acid isolates BR 12 and BR 17 with limited sucrose. The material in this study was probiotic isolates of Lactic Acid Bacteria (LAB) BR 12 and BR 17 which were isolated from broiler chicken meat, determined media, and sucrose. The method used was growed probiotic LAB isolates in the media determined with a sukora concentration that wasdifferent from 0-1%. The results showed that the Lineweaver-Burk equation of the LAB probiotic BR 12 was y = 3.503x + 10.10. the maximum speed (μmax) LAB probiotic BR 12 was0.0075 hour-1 and the constant Michaelis-Menten (KS) was 0.264g / 100 ml. Lineweaver-Burk equation of LAB probiotic BR 17 was y = 2.809x + 11.40. The maximum speed (μmaks) of LAB probiotics BR 17 was 0.088 / hour and the Michaelis-Menten constant (Km) = 0.2464 g / 100ml. The conclusion of this study was that the Lineweaver-Burk equation BR 12 was different from BR 17. The value of μmax and Ks BR 17 was lower than BR 12.