Research Article
Effect of Alkaline Pre-treatment on Fermentable Sugar Yield of Ethiopian Bamboo (Yushania Alpine)
@INPROCEEDINGS{10.1007/978-3-030-80621-7_4, author={Netsanet Alemayehu and Yalew W/Amanuel and Nigus Gabbiye}, title={Effect of Alkaline Pre-treatment on Fermentable Sugar Yield of Ethiopian Bamboo (Yushania Alpine)}, proceedings={Advances of Science and Technology. 8th EAI International Conference, ICAST 2020, Bahir Dar, Ethiopia, October 2-4, 2020, Proceedings, Part I}, proceedings_a={ICAST}, year={2021}, month={7}, keywords={High land bamboo Pretreatment process Delignification Fermentable sugar}, doi={10.1007/978-3-030-80621-7_4} }- Netsanet Alemayehu
Yalew W/Amanuel
Nigus Gabbiye
Year: 2021
Effect of Alkaline Pre-treatment on Fermentable Sugar Yield of Ethiopian Bamboo (Yushania Alpine)
ICAST
Springer
DOI: 10.1007/978-3-030-80621-7_4
Abstract
In the recent years, bioethanol derived from lignocellulosic biomass as an alternative to the conventional fuels is becoming a focus of many researchers. In this study, bamboo is used as a feedstock for the production of bioethanol via alkaline pretreatment followed by, enzymatic and acid hydrolysis process. Specifically, the focus is mainly on the investigation of the effect of alkaline pretreatment on the yield of total fermentable sugar from bamboo (yushania alpine) biomass using sodium hydroxide as a catalyst. Alkali concentration, pretreatment temperature, and reaction time were varied in the alkali pretreatment experiments. Following the pretreatment, enzymatic and dilute sulfuric acid hydrolysis of the pretreated bamboo were carried out separately to measure the success of the alkaline pretreatment in improving the total reducing sugar (fermentable sugar) yields. The composition of raw and pretreated bamboo were characterized using NREL and ASTM protocols. The total reducing sugar concentrations after hydrolysis were determined by DNSA method. It was found that, the raw bamboo has a composition of 42.45% cellulose, 23.6% hemicelluloses, and 32.4% Lignin. The alkali pretreatment resulted in a maximum lignin removal of 35.5% at temperature of 60 °C, contact time of 50 min, and alkali concentration of 4.5% w/v, which leads to an increment of cellulose fraction to 64.93% and reduction of lignin to 20.8%. A maximum total reducing sugar yield of 206.3 mg/g and 351.9 mg/g were obtained for pretreated bamboo by using enzymatic and dilute sulfuric acid hydrolysis, respectively.
