Research Article
Antiviral Activity ofAloe pirottaeA. Berger Root Extracts Against Influenza A and B Viruses, Picornaviruses and Dengue Virus: An Endemic Plant Species of Ethiopia
@INPROCEEDINGS{10.1007/978-3-030-80621-7_2, author={Akalu Terfa and G. Anuradha and Hailemicahel Tesso and Young Sik Jung and Sang Un Choi and Sang Ho Lee and J. Sreekantha Kumar}, title={Antiviral Activity ofAloe pirottaeA. Berger Root Extracts Against Influenza A and B Viruses, Picornaviruses and Dengue Virus: An Endemic Plant Species of Ethiopia}, proceedings={Advances of Science and Technology. 8th EAI International Conference, ICAST 2020, Bahir Dar, Ethiopia, October 2-4, 2020, Proceedings, Part I}, proceedings_a={ICAST}, year={2021}, month={7}, keywords={Antiviral Influenza A and B virus Picornavirus Aloe pirottae}, doi={10.1007/978-3-030-80621-7_2} }- Akalu Terfa
G. Anuradha
Hailemicahel Tesso
Young Sik Jung
Sang Un Choi
Sang Ho Lee
J. Sreekantha Kumar
Year: 2021
Antiviral Activity ofAloe pirottaeA. Berger Root Extracts Against Influenza A and B Viruses, Picornaviruses and Dengue Virus: An Endemic Plant Species of Ethiopia
ICAST
Springer
DOI: 10.1007/978-3-030-80621-7_2
Abstract
This study is aimed to evaluate the antiviral studies ofAloe pirottaeroot extracts in Vitro on Influenza A and B viruses, Picornaviruses, and Dengue virus. During this study, crude methanol extract (MeOH) and its organic solvent fractions including n-hexane (HxF), chloroform (CHF), ethyl acetate (EAF) and n-butanol (BuF) ofAloe pirottaeroot in different concentrations were prepared and evaluated for a cytopathic effect (CPE) inhibitory assay for influenza virus and picornavirus and an immunofluorescence assay (IFA) for dengue virus. In vitro test revealed thatAloe pirottaeroot extracts have shown important effects in reducing both A and B influenza viruses, HRV B14 and EV-71(H) in MDCK cells, and DENV-2 replication in Vero cells. EAF exhibit more potential inhibitory activity with selectivity index >7.2 >17.8 and >8.5 than positive controls AMT with selectivity index not determined (ND), >47.6 and ND and RBV with selectivity index >2.9, >5.3 and >5 against PR8, HK and Lee, respectively. EAF exhibits the strongest anti-HRV B14 and anti-EV-71(H) activity in MDCK cells. Crude MeOH extract, EAF and BuF at both concentrations exhibit strong inhibition of DENV-2 induced CPE where as CHF and HxF at 20 µg/ml exhibit strong inhibition of DENV-2 production. The findings show significant inhibitory effect ofAloe pirottaeroot on influenza A and B viruses, HRV B14 and EV-71(H), and DENV-2 replication.
