Research Article
Resolution-Improvement of Confocal Fluorescence Microscopy via Two Different Point Spread Functions
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@INPROCEEDINGS{10.1007/978-3-030-63083-6_6, author={Xuanhoi Hoang and Vannhu Le and MinhNghia Pham}, title={Resolution-Improvement of Confocal Fluorescence Microscopy via Two Different Point Spread Functions}, proceedings={Industrial Networks and Intelligent Systems. 6th EAI International Conference, INISCOM 2020, Hanoi, Vietnam, August 27--28, 2020, Proceedings}, proceedings_a={INISCOM}, year={2020}, month={11}, keywords={High-resolution Confocal fluorescence microscopy Image processing}, doi={10.1007/978-3-030-63083-6_6} }- Xuanhoi Hoang
Vannhu Le
MinhNghia Pham
Year: 2020
Resolution-Improvement of Confocal Fluorescence Microscopy via Two Different Point Spread Functions
INISCOM
Springer
DOI: 10.1007/978-3-030-63083-6_6
Abstract
In this paper, we propose a new method to obtain the improvement of lateral axial resolution of confocal fluorescence microscopy. In this method, we employ two different beams to illuminate the sample: (1) the Gaussian beam; (2) the donut beam. Two different images are produced from these two illumination beams. A higher resolution image is generated by a multi-relationship between these two image. A set of simulation and experimental results are employed to compare the effectiveness of proposed method with the traditional confocal fluorescence microscopy. These results demonstrated that our method can be employed to achieve the resolution-enhancement of confocal fluorescence microscopy.
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